증기폭쇄 방법으로 국내산 대나무줄기에서 추출된 리그닌의 자외선 차단효과

• Published in: KSBB Journal Vol. 32; no. 4; pp. 342 - 351
• Main Authors: 양승화(Seung-Hwa Yang), 최문희(Moon-Hee Choi), 신현재(Hyun-Jae Shin)
• Format: Journal Article
• Language: Korean
• Published: 한국생물공학회 2017
• Subjects: 생물공학; domestic bamboo stem; DPPH; steam explosion; sun protection factor; lignin; antioxidant activity; ABTS
• ISSN: 1225-7117; 2288-8268
• DOI: 10.7841/ksbbj.2017.32.4.342

Bamboo trees have high economic values because of rapid growth rate, rich biomass components and good health effects. Among several parts of bamboo tree, bamboo stem contains a considerable amount of cellulose and lignin, and a little amount of minerals, silica, and etc. In this study, domestic bamboos (Phyllostachys bambusoides (1) and Phyllostachys nigra var. henosis (2) lignins were prepared by steam explosion (temperature $210^{\circ}C$, 2 MPa) and organosolv pretreatment. The results showed that steam explosion lignin (SEL1, SEL2) had a higher yield (12% and 21%). Characteristics of lignin structures were confirmed by FT-IR, and $^1H$-, $^{13}C$-, and 2D HSQC NMR. SEL1 and SEL2 were the typical GHS-type lignins and their bonds units were predominantly ${\beta}$-O-4'linkages. Antioxidant effects were assayed by DPPH and ABTS radical scavenging activity using ascorbic acid as a standard. The antioxidant activity of SEL1 was the best, and $IC_{50}$ value of $383.89{\pm}25.35{\mu}g/mL$ and $131.78{\pm}0.99{\mu}g/mL$, respectively. Cytotoxic concentration levels of all lignin samples were > $100{\mu}g/mL$ based on MTT assay. To confirm the UV protection effect of lignin, SEL1 was added to commercial sunscreen (SPF 15) and moisturizing cream (no SPF) respectively. Addition of 10 wt% lignin to commercial sunscreen increased 45% of UV absorbance higher than moisturizing cream after 2 h of UV (UVA+UVB) radiation. The result of lignin addition has shown synergistic effects in UV protection and antioxidant effect.