Anti-inflammatory Effect of Flower Bud and Fruit of Sweet Persimmon, Diospyros kaki T

• Published in: Journal of experimental & biomedical sciences Vol. 26; no. 2; pp. 85 - 92
• Main Authors: Park, Yeo Ok, Lee, Jeong Ah, Park, Seong Moon, Ha, Min Hee, Joo, Woo Hong, Kim, Dong Wan
• Format: Journal Article
• Language: Korean
• Published: 대한의생명과학회 2020
• Subjects: NF-κB; Sweet persimmon; Anti-oxidant; Anti-inflammatory; Wnt/β-catenin; Wnt/{\be ta}$-catenin; NF- ${\kappa}B
• ISSN: 1738-3226; 2288-7415

Various beneficial effects of sweet persimmon (Diospyros kaki T.) including anti-oxidation, anti-bacteria and viruses, anti-allergy were widely reported previously. However, the anti-inflammatory effect and its molecular mechanisms are not clear. In this study, the anti-inflammatory effect of the extracts of flower bud and fruit of sweet persimmon was investigated in LPS-treated RAW264.7 cells. Both extracts of flower bud and fruit showed strong inhibitory effect on the LPS-induced NF-κB activation. IκBα, the inhibitor of NF-κB, was increased and the expressions of NF-κB target genes, COX-2 and iNOS, were suppressed by the treatment with the extracts of flower bud and fruit. The expressions of pro-inflammatory cytokines, IL-1β, IL-6, TNF-α were also suppressed by the extracts. In addition, the LPS-induced wnt/β-catenin pathway and its related gene expressions including cyclin D1, wnt 3a, wnt 5a were suppressed by the extracts. The extracts also showed anti-oxidant activity and suppressive effect on the LPS-induced apoptosis of RAW264.7 cells. These results suggest that the flower bud and fruit of sweet persimmon display strong anti-inflammatory effect through inhibiting the pro-inflammatory signaling pathways in the cells.