‘매향’ 딸기의 조직배양 시 coconut water 처리에 따른 기내 증식효율 향상

• Published in: Journal of plant biotechnology Vol. 47; no. 3; pp. 242 - 247
• Main Authors: 김혜진(Hye Jin Kim), 최미자(Mi Ja Choi), 이종남(Jong Nam Lee), 서종택(Jong Taek Suh), 김기덕(Ki Deog Kim), 김율호(Yul Ho Ki
• Format: Journal Article
• Language: Korean
• Published: Korean Society for Plant Biotechnology 30.09.2020; (사)한국식물생명공학회; 한국식물생명공학회
• Subjects: 농학; morphological variant; genetic variation; Fragaria × ananassa; proliferation; Fragaria ${\times}$ ananassa
• ISSN: 1229-2818; 2384-1397
• DOI: 10.5010/JPB.2020.47.3.242

This experiment was performed to determine the concentration of coconut water that is most effective in promoting the growth of 'Maehyang' strawberry in tissue culture. Cultivars in this experiment consisted of 'Maehyang' grown in the presence of 0, 20, 40, 60, 80, 100 ml·L-1 of coconut water which was added to a medium mixed with BA 0.5 mg·L-1 and IBA 0.1 mg·L-1. Morphological variation tests and SSR detection with coconut water were conducted to determine variations in proliferation. The proliferation rate of 'Maehyang' strawberry improved with the coconut water treatment compared to non-treatment. In particular, the proliferation rate with 100 ml·L-1 coconut water treatment increased by about 4 times. When 'Maeyang' was treated with 100 ml·L-1 of coconut water, two morphological variants occurred without genetic variation. Therefore, the results suggest that 100 ml·L-1 of coconut water can be used to mass-produce "Maeyang" strawberry without causing genetic variations. 본 실험은 딸기 조직배양 시 증식률 향상을 위해 coconut water의 적정처리 농도 구명하고자 실시하였다. 본 실험에 사용된 시험 품종은 '매향'이며, BA 0.5 mg·L-1와 IBA 0.1 mg·L-1을 혼합처리한 배지에 coconut water를 0, 20, 40, 60, 80, 100 ml·L-1로 첨가하여 실험하였다. Coconut water 처리 시 형태적 변이검정과 SSR 검정을 통해 변이 발생 유무를 확인하였다. '매향'은 무처리에 비해 coconut water 처리에서 증식률이 향상되었으며, 특히 coconut water 100 ml·L-1 처리에서 증식률이 약 4배 향상되었다. '매향'에 coconut water 100 ml·L-1 처리 시 형태적 변이체가 2개체 발생하였으나 유전적 차이는 보이지 않았다. 따라서 '매향' 조직배양 시 coconut water 100 ml·L-1를 처리하면 유전적 변이없이 대량증식이 가능할 것으로 판단된다.