LPS로 자극한 RAW264.7 세포에서 크랜베리 폴리페놀 분획물의 항산화 효과

• Published in: Han'guk Sikp'um Yŏngyang Kwahakhoe chi Vol. 44; no. 8; pp. 1241 - 1247
• Main Authors: 정하나(Hana Jung), 이기욱(Kiuk Lee), 황금택(Keum Taek Hwang), 곽호경(Ho-Kyung Kwak)
• Format: Journal Article
• Language: Korean
• Published: 한국식품영양과학회 2015
• Subjects: 식품과학; cranberry; antioxidant; polyphenol; inflammation
• ISSN: 1226-3311; 2288-5978

The objective of this study was to determine antioxidant properties of polyphenol fractions of cranberry powder employing lipopolysaccharides (LPS)-stimulated RAW264.7 macrophage cells. Ethyl acetate fraction (EF) and methanol fraction (MF) of cranberry powder were prepared using a C18 Sep-Pak cartridge. When cells were treated with LPS for 20 h, intracellular reactive oxygen species (ROS) and DNA damage significantly increased. In cells pre-treated with EF, MF, and total fraction (TF: combining EF and MF), significant reductions of intracellular ROS were observed. The tested fractions reduced LPS-induced DNA damage measured by Hoechst staining. In addition, LPS-induced DNA oxidation was attenuated when cells were pre-treated with TF and MF. However, there was no significant difference in LPS-induced superoxide dismutase activity. 본 연구의 목적은 LPS로 자극한 RAW264.7 세포를 이용하여 크랜베리에서 분리한 폴리페놀 분획물의 항산화능을 분석하는 것이었다. 크랜베리 분말의 에틸 아세테이트 분획(EF)과 메탄올 분획(MF)은 C18-Sep-Pak 카트리지를 사용하여 분리했다. 20시간 동안 LPS로 자극한 RAW264.7 세포는 활성산소종(ROS)과 DNA 손상이 유의하게 증가하였으며, EF, MF 및 TF(EF+MF)로 전처리하고 LPS로 자극한 RAW264.7 세포에서는 ROS와 DNA 손상이 유의적으로 감소했다. 그러나 superoxide dismutase의 활성에는 차이가 없었다.