KH-304 투여가 흰쥐 음경조직의 Nitric Oxide Synthase활성 및 Erectile dysfunction에 미치는 영향

• Published in: 동의생리병리학회지 Vol. 20; no. 3; pp. 680 - 684
• Main Authors: 이은정(Eun Jeong Lee), 이현지(Hyun Ji Lee), 김희석(Hee Seok Kim), 황성연(Sung Yeoun Hwang)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2006
• Subjects: 한의학; COMP; Intracavernous pressure; Erectile dysfunction; NOS (Nitric Oxide Synthase)
• ISSN: 1738-7698; 2288-2529; 2283-2529

This study was designed to investigate effects of KH-304 in improving erectile dysfunction (ED), particularly in terms of nitric oxide (NO)-cGMP pathways. After oral administration of the KH-304 water extract, 1OOmg, 300mg, 500mg or 700mg per 1 kg of Dody weigh for 10days, We examined the expression and activity of two enzyme: neuronal NO synthase (nNOS), endothelial NO synthase (eNOS) and that act upon the major NO-cGMP signaling pathway in penile tissue. Effect of KH-304 on COMP degradation was also examined using bovine vascular smooth muscle cells pretreated with an NO donor, S-nitroso-N-Acetylpenicillamine (SNAP), Also, it examined the endothelial NO synthase (eNOS) for seaching effecting period (100mg, 300mg/kg for 10 and 30days) and peak intracavernous pressures (ICPS) in penile tissues rabbit copus cavernosum contracted by 10-6 M phenylephrine. The severely reduced peak intracavernous pressures (ICPS) in penile tissues were restored completely after KH-304 treatment, and KH-304 treatment significantly made the latency period earlier. Furthermore, the penile expression levels of nNOS, eNOS dependent NOS activities and COMP concentrations were increased significantly in the KH-304 100, 300mg treated rats. These results suggest that KH-304 with high expression of NOS may be useful in erectile dysfunction.