수치에 따른 결명자 주요 Anthraquinone의 함량분석

• Published in: 생약학회지 Vol. 45; no. 3; pp. 200 - 208
• Main Authors: 김병수(Byoung Soo Kim), 황석연(Seock Yeon Hwang), 신현규(Hyeun Kyoo Shin), 김정훈(Jung Hoon Kim), 서창섭(Chang Seob Seo)
• Format: Journal Article
• Language: Korean
• Published: 한국생약학회 2014
• Subjects: 약학; Emodin; Cassiae Semen; Physcion; Processing method; Aurantio-obtusin; Chrysophanol
• ISSN: 0253-3073; 2288-9299

In this study, we performed quantification determination of four major components including aurantio-obtusin, emodin, chrysophanol, and physcion in the 70% ethanol extracts of non-processed Cassiae Semen and processed Cassiae Semen using a high-performance liquid chromatography coupled with photodiode array detector. The analytical column for separation of the 4 constituents used a Gemini $C_{18}$ column kept at $40^{\circ}C$ by the gradient elution with 1.0% (v/v) acetic acid in water and 1.0% (v/v) acetic acid in acetonitrile as mobile phase. The flow rate was 1.0 mL/min and the injection volume was $10{\mu}L$. The amount of aurantio-obtusin, emodin, chrysophanol, and physcion in non-processed Cassiae Semen were 0.07%, 0.02%, 0.25%, and 0.10%, respectively. The amount of aurantio-obtusin, emodin, chrysophanol, and physcion in processed Cassiae Semen were 0.04-0.14%, 0.01-0.03%, 0.02-0.42%, and 0.01-0.24%, respectively. Consequently, the optimal processing condition of Cassiae Semen for the improvements of amounts of four anthraquinone compounds was obtained by roasting at $240^{\circ}C$ for 15 min.