상기생 물추출물이 대식세포 활성화와 Th1 반응에 미치는 효과

• Published in: 동의생리병리학회지 Vol. 22; no. 1; pp. 171 - 175
• Main Authors: 신혜영(Hye Young Shin), 장문희(Wen Ji Zhang), 김윤철(Youn Chul Kim), 윤용갑(Yong Gab Yun), 박현(Hyun Park)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2008
• Subjects: 한의학; Loranthi Ramulus $TNF-{\alpha}; TLR4; Nitric oxide
• ISSN: 1738-7698; 2288-2529; 2283-2529

In the recently, increased concern has been focused on the pharmacology and clinical utility of herbal extracts and derivatives as a drug or adjunct to chemotherapy and immunotherapy. Here we investigated the effect of the water extract of Loranthi Ramulus (LR) in production of inflammatory mediators and expression of toll-like receptor (TLR)-4, CD14 from peritoneal macrophage. We assayed the effect of LR water extract in cell proliferation in vitro and Th1/Th2 cytokine level in vivo. In peritoneal macrophages, water extract of LR water extract increased the production of Nitric oxide (NO) and $TNF-{\alpha}$. Also, LR water extract increased Con A-induced cell proliferation and IgG1, IgG2a level in serum. However, i.p. injection of water extract of LR water extract did not affect the level of $TNF-{\alpha}$, $IFN-{\gamma}$, IL-2, IL-4 and IL-5 in serum of mice. These studies indicate that LR water extract induces macrophage activation and suggest the possible use of LR water extract in macrophage-based immunotherapies.