백서의 배양 골아세포와 파골세포에 대한 산화적 손상과 Glutamate 수용체 길항제의 영향

• Published in: 동의생리병리학회지 Vol. 17; no. 4; pp. 996 - 1001
• Main Authors: 박승택(Seung Taeck park), 전승호(Seung Ho Jeon), 이병찬(Byung Chan Lee)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2003
• Subjects: 한의학; Osteoblast; Osteoclast; Glutamate receptor
• ISSN: 1738-7698; 2288-2529; 2283-2529

It is well known that oxidative stress of reactive oxygen species(ROS) may be a causative factor in the pathogenesis of bone disorder. The purpose of this study was to evaluate the cytotoxicity of oxidative stress. Cell viability by MTS assay or INT assay, activity of glutathione peroxidase(GPx), lipid peroxidation(LPO) activity and cell viablity. And also protctive effect of glutamate receptors against ROS-induced osteotoxicity was examined by protein synthesis, alkaline phosphatase (ALP) activity and lactate dehydrogenase (LDH) activity in cultured rat osteoblasts and osteoclasts. XO/HX decreased cell viability and GPx activity, protein synthesis and ALP activity, but increased LPO activity and LDH activity. In the protective effect, N-methyl-D-aspartate (NMDA) receptor antagonists or AMPA/kainate receptor antagonists such as D-2-amino-5-phosphonovaleric acid (APV), 7-chlorokynurenic acid (CKA), 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and 6,7-dinitroquinoxaline-2,3-dione (DNQX), NMDA receptor antagonists but AMPA/kainate receptor antagonists showed protective effect on xanthine oxidase (XO) and hypoxanthine (HX) in these cultures by the increse of protein synthesis, ALP activity.