어성초 용매추출물과 메탄올 분획물의 암세포주에 대한 세포독성

• Published in: 동의생리병리학회지 Vol. 17; no. 5; pp. 1288 - 1292
• Main Authors: 이정호(Jeong Ho Lee), 백승화(Seung Hwa Baek), 임진아(Jin A Lim), 천현자(Hyun Ja Chun), 이기남(Ki Nam Lee)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2003
• Subjects: 한의학; Cytotoxic effect; MTT assay; Houttuynia cordata THUNB
• ISSN: 1738-7698; 2288-2529; 2283-2529

This study was carried out to evaluate cytotoxic effects of Houttuynia cordata T/sub HUNB/ extracts on A549 (lung cancer), MDA-MB231 (breast cancer), SNU-C4 (colon cancer) and B16 (mouse melanoma) cell lines. We have determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. The 150 ㎍/㎖ concentration of methanol extract (63.81 %) of Houttuynia cordata T/sub HUNB/ was shown significantly antitoxic activity on A549 cell lines. The order of cytotoxicity fractions of methanol from Houttuynia cordata T/sub HUNB/ extracts against cancer cell lines in vitro is as follows : hexane fraction layer > chloroform fraction layer > ethyl acetate fraction layer > buthanol fraction layer > water fraction layer. These results suggest that the hexane fraction of methanol extract from Houttuynia cordata T/sub HUNB/ extract may be a valuable choice for the development of antitumor agents.