황금 물추출물의 당화종말산물로 유도한 THP-1 세포의 염증반응 억제효과

• Published in: 동의생리병리학회지 Vol. 26; no. 3; pp. 273 - 280
• Main Authors: 박평범(Pyeong Beom Park), 김민준(Min Jun Kim), 신경호(Kyoung Ho Shin), 이광규(Kwang Gyu Lee), 이창현(Chang Hyun Lee), 이상룡(Sang Ryong Lee), 하기태(Ki Tae Ha), 정한솔(Han Sol Jeong)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2012
• Subjects: 한의학; THP-1 cells; scutellaria baicalensis georgi; advanced glycation end products; AGE
• ISSN: 1738-7698; 2288-2529; 2283-2529

Scutellaria baicalensis Georgi, which has been known to be able to clear away heat and remove dampness, was used for febrile disease. It is now clear that Advanced glycation end products (AGEs) play major roles in the pathogenesis of diabetic complications such as atherosclerosis. In this study, we examined whether Scutellaria baicalensis Georgi suppress the AGE mediated inflammatory responses in the THP-1 cells. AGE treatment increased the gene expression of pro-inflammatory cytokines such as tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-$1{\beta}$ (IL-$1{\beta}$), monocyte chemotactic protein-1 (MCP-1) and cyclooxygenase-2 (COX-2). Reverse transcriptase-polymerase chain reaction and Western blot analysis revealed that S. baicalensis had inhibitory effects on the expression of pro-inflammatory genes and protein levels in AGE-treated THP-1 cells. S. baicalensis had also reduced the production of ROS in the AGE-treated THP-1 cells. These results suggest that S. baicalensis has inhibitory effects for the development of diabetic vascular complication.