柴胡가 OVA로 유발된 BALB/c mice의 알레르기성 비염 모델에 미치는 영향

• Published in: 동의생리병리학회지 Vol. 29; no. 6; pp. 467 - 474
• Main Authors: 임정민(Jeong-Min Lim), 허준(Jun Heo), 이성엽(SungYub Lee), 김재현(Jae-Hyun Kim), 이비나(Bina Lee), 민주희(Ju-Hee Min), 김은영(Eun-Young Kim), 정혁상(Hyuk-Sang Jung), 손영주(Youngjoo Sohn)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2015
• Subjects: 한의학; BALB/c mice; Allergic Rhinitis; Bupleuri Radix
• ISSN: 1738-7698; 2288-2529; 2283-2529
• DOI: 10.15188/kjopp.2015.12.29.6.467

Allergic rhinitis is a growing tendency to increase. Following this tendency, allergic rhinitis is lively studied also in Korean oriental medicine. Bupleuri Radix (BR) has been used for many inflammatory diseases, but experimental backgrounds are not enough to treat allergic rhinitis. So in this study, effects of BR on OVA-induced allergic rhinitis model of BALB/c mice were examined. Thirty two BALB/c mice are equally devided into four groups; control group, OVA group, OVA+BR group, OVA+Cet group. The OVA, OVA+BR and OVA+Cet groups were induced allergic rhinitis by sensitizing to OVA. And then saline solution included BR (10.6 mg/kg body weight) was administered to the OVA+BR group orally. The number of nasal rubbing and nasal sneezing was evaluated for 10 days and later serological and histological changes were analyzed. Serological analysis included the serum levels of cytokines and chemokines (IL-4, IL-5, IL-12, MCP-1, MIP-2), total IgE and OVA-specific IgE levels in serum. Histological analysis included thickness of nasal septum, eosinophil counts changes of nasal mucosa, infiltration of eosinophil in nasal mucosa and histological changes of nasal mucosa. The number of nasal rubbing and nasal sneezing was significantly decreased in the OVA+BR group. The serum levels of IL-4, IL-5, MCP-1, MIP-2 were significantly decreased in the OVA+BR group but the serum levels of IL-1β had not significance. Total IgE and OVA-specific IgE levels in serum were decreased in the OVA+BR group, but total IgE levels in serum had only significance. Thickness of nasal septum, eosinophil counts of nasal mucosa and infiltration of eosinophil in nasal mucosa were significantly decreased in the OVA+BR group. From the results of this study, we think that BR has an effect on improvement of allergic rhinitis by improving nasal rubbing and nasal sneezing, reducing histological changes of nasal mucosa and infiltration of eosinophil in nasal mucosa, inhibiting increasing of the serum levels of cytokines, chemokines and total IgE.