상기생 추출물이 파골세포 분화와 골흡수 억제에 미치는 효과

• Published in: 동의생리병리학회지 Vol. 27; no. 4; pp. 431 - 436
• Main Authors: 백종민(Jong Min Baek), 김주영(Ju Young Kim), 이명수(Myeung Su Lee), 정우진(Woo Jin Jeung), 문서영(Seo Young Moon), 전병훈(Byung Hoon Jeon), 오재민(Jae Min Oh), 최민규(Min Kyu Choi)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2013
• Subjects: 한의학; Bone resorption; Taxillus chinenesis; Osteoporosis; Osteoclast; Differentiation
• ISSN: 1738-7698; 2288-2529; 2283-2529
• DOI: 10.15188/kjopp.2013.08.27.4.431

Bone homeostasis is maintained by co-ordination of bone-resorbing osteoclasts and bone-forming osteoblasts. Imbalance between osteoclasts and osteoblasts leads to many bone diseases such as osteoporosis, rheumatoid arthritis. Taxillus chinensis is a herb that has been widely used to improve bone health. However, the effect and mechanism of Taxillus chinensis extract on osteoclast differentiation and bone resportion has been unknown. Thus, We investigated the effect of Taxillus chinensis on expression of receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation and bone resorption. Also, the action of Taxillus chinensis on mechanisms relating to osteoclast differentiation was studied. In this results, we identified that Taxillus chinensis significantly inhibited RANKL-induced osteoclast differentiation and bone resportion. Moreover, Taxillus chinensis was suppressed the activation of NF-${\kappa}B$ in bone marrow macrophage treated RANKL and M-CSF. Taxillus chinensis was down-regulated the mRNA expression of c-Fos, nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP). The cell adhesion-related molecules such as integrin ${\alpha}v$ and integrin ${\beta}3$, and the filamentous actin (F-actin) rings of mature osteoclasts-related molecules such as dendritic cell-specific transmembrane preotein (DC-STAMP) and cathepsin K are also suppressed. Taken together, these results indicated that Taxillus chinensis will be a good candidate to treat osteoclast-mediated bone diseases.