Next generation sequencing 방법을 이용한 적육계 복숭아와 백육계 복숭아의 전사체 분석

• Published in: Journal of plant biotechnology Vol. 39; no. 4; pp. 273 - 280
• Main Authors: 김세희(Se Hee Kim), 남은경(Eun Young Nam), 조강희(Kang Hee Cho), 신일섭(H Sheob Shin), 김현란(Hyun Ran Kim), 황해성(Hae Seo
• Format: Journal Article
• Language: Korean
• Published: Korean Society for Plant Biotechnology 31.12.2012; (사)한국식물생명공학회; 한국식물생명공학회
• Subjects: 농학
• ISSN: 1229-2818; 2384-1397

Differences of gene expression between red flash peach cultivar and white flash peach cultivar were investigated by Nest-generation sequencing (NGS). EST from the red flash peach cultivar and white flash peach cultivar were selected for nucleotide sequence determination and homology searches. The levels of transcripts coding for proteins involved in pathogenesis related proteins, temperature stress, ethylene signal pathway were significantly higher in white flash peach cultivar than in red flash peach cultivar. On the other hand, the up-regulation of proteins involved in anthocyanin and flavonol biosynthesis and protein degradation and sorbitol metabolism were observed in red flash peach cultivar. Chalcone synthase was preferentially expressed in the red flesh peach cultivar, agreeing with the accumulation of anthocyanin and expression of other previously identified genes for anthocyanin biosynthesis. Anthocyanin pathway related genes CHS, F3H, DFR, LDOX, UFGT differentially expressed between red flash peach cultivar and white flash peach cultivar. These results suggest that red flash peach cultivar and white flash peach cultivar have different anthocyanin biosynthesis regulatory mechanisms.