프로바이오틱스 개발을 위한 홍삼 전분을 활용한 유산균 배양

• Published in: 東아시아食生活學會誌 Vol. 23; no. 6; pp. 818 - 826
• Main Authors: 김영수(Yeong-Su Kim), 이환(Hwan Lee), 김도연(Do-Yeon Kim), 김소영(So-Young Kim), 이완규(Wan-Kyu Lee), 이상명(Sang-Myeong Lee), 박종대(Jong-Dae Park), 손미례(Mi-Yae Shon)
• Format: Journal Article
• Language: Korean
• Published: 동아시아식생활학회 2013
• Subjects: 생활과학; Lactic acid bacteria; red ginseng starch; gensenoside; red ginseng starch medium
• ISSN: 1225-6781; 2288-8802

To reduce the production cost of Lactobacillus, discarded red ginseng starch was collected from a factory of red ginseng extract in order to develop the Lactobacillus culture medium. According to the analysis of the gensenoside composition of red ginseng starch, the total gensenoside content of starch was 2.73 mg/g, and the gensenoside $Rb_1$, $Rb_2$ and $Rg_3$ contents were 0.1, 0.29 and 0.52 mg/g, respectively. For the preparation of the liquid media, red ginseng starch was added at rates of 0, 5, 10 and 20%. Further, Lactobacillus plantarum 15357 and Leuconostoc mesenteroides sub sp. strains were then inoculated to these prepared broths. With the red ginseng starch medium, the growth rates ($1.42{\times}10^7$ and $2.96{\times}10^{10}$ CFU/mL) and the final cell concentrations were higher than the MM medium ($1.0{\times}10^7$ CFU/mL). The optimal concentration of red ginseng starch and yeast extract as a medium were 20% and 10 g/L, respectively. Under these conditions, the cell mass of L. plantarum 15357 and L. mesenteroides sub sp. reached $5.11{\times}10^{10}$ and $8.17{\times}10^{10}$ CFU/mL. These results show a great possibility for the utilization of red ginseng starch as economic medium sources in the production of cell mass of lactic acid bacteria. This is the first trial of development of economic LAB growth medium using discarded red ginseng starch.