파골세포 분화에 복령 추출물이 미치는 영향

• Published in: 동의생리병리학회지 Vol. 26; no. 3; pp. 320 - 324
• Main Authors: 천윤희(Yoon Hee Cheon), 곽성철(Seong Cheoul Kwack), 오재민(Jaemin Oh), 최민규(Min-Kyu Choi), 김정중(Jeong Joong Kim), 곽한복(Han Bok Kwak), 이명수(Myeung Su Lee), 전병훈(Byung Hoon Jeon), 문서영(Seo Young Moon)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2012
• Subjects: 한의학; RANKL; hoelen; osteoporosis; osteoclast
• ISSN: 1738-7698; 2288-2529; 2283-2529

Osteoporosis is an important public health issue in postmenopausal women. It is a major public health concern and is widely believed that osteoporosis results from imbalance between bone resorption and bone formation. Recently natural products from plants have been extensively studied as therapeutic drugs to treat and prevent various diseases. Hoelen (scientific name, Poria cocos) is a mushroom that is used in traditional Chinese medicine. Hoelen exhibits anti-inflammatory activity and has a protective effect on tumor progression. However, the effect of hoelen in osteoclast differentiation remains unknown. Thus, we examined the effect of hoelen in receptor activator of nuclear factor-${\kappa}B$ ligand (RANKL)-induced osteoclast differentiation. Hoelen significantly inhibited RANKL-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) in dose dependent manner without toxicity. Also, we showed that hoelen significantly inhibited the mRNA expression of tartrate-resistant acid phophatase (TRAP) and nuclear factor of activated T cells 1 (NFATc1) in BMMs treated with RANKL. In Particular, hoelen greatly inhibited the protein expression of NFATc1. Ectopic expression of NFATc1 partially reverses hoelen-mediated inhibition of osteoclast differentiation. Taken together, our results demonstrated that hoelen may be useful treatment option of bone-related disease such as osteoporosis, reumatoid arthritis, and periodontitis.