조릿대의 잎과 줄기 추출물 분획의 염증 및 비만 억제 효과 비교

• Published in: 동의생리병리학회지 Vol. 30; no. 4; pp. 229 - 235
• Main Authors: 하유빈(You Bin Ha), 박재형(Jae Hyoung Park), 장준우(Jun Woo Jang), 임동우(Dong Woo Lim), 김재은(Jai Eun Kim)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2016
• Subjects: 한의학; preadipocyte differentiation; iNOS; COX-2; Sasa borealis; ROS; fraction
• ISSN: 1738-7698; 2288-2529; 2283-2529
• DOI: 10.15188/kjopp.2016.08.30.4.229

Sasa borealis has long been used in folk remedies and traditional Korean medicine for analgesic, sedative and anticarcinogenic purposes. To excavate novel natural compounds for treating obesity, we separated leaf and stem and fractionated. We screened phenolic contents, anti-oxidative and anti-inflammatory properties with the fractions. Dichloromethane (DCM) and ethyl acetate (EA) fraction from both leaf and stem exhibited high anti-oxidative properties demonstrated by DPPH, ABTS assay in accordance with their phenolic contents. In addition, DCM and EA fraction from leaf inhibited nitric oxide (NO) production as well as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in Raw 264.7 cell. Finally, DCM and EA fraction from both leaf and stem significantly inhibited 3T3-L1 preadipocyte differentiation and decreased intracellular lipid level. These results suggest that DCM and EA fraction from Sasa borealis may contain therapeutic agent for obesity by attenuating inflammation and oxidative stress.