머위 분획물의 In Vitro 라디칼 소거능 및 신경세포의 산화적 스트레스 보호 효과

• Published in: 생약학회지 Vol. 45; no. 2; pp. 147 - 153
• Main Authors: 왕천(Qian Wang), 이아영(Ah Young Lee), 최지명(Ji Myung Choi), 이동구(Dong Gu Lee), 김현영(Hyun Young Kim), 이상현(Sanghyun Lee), 조은주(Eun Ju Cho)
• Format: Journal Article
• Language: Korean
• Published: 한국생약학회 2014
• Subjects: 약학; Oxidative stress; Reactive oxygen species; Hydrogen peroxide; C6 glial cells; Petasites japonicus
• ISSN: 0253-3073; 2288-9299

This study was focused on the evaluation of radical scavenging effect and the protective activity against oxidative stress of the extract and fractions from Petasites japonicus. P. japonicus was extracted with methanol and then fractionated into 4 fractions [n-butanol, ethyl acetate (EtOAc), methylene chloride, and n-hexane]. The extract and fractions showed strong 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Among all the fractions, particularly, the EtOAc fraction showed the strongest effect with the $IC_{50}$ value of $0.02{\mu}g/ml$. In addition, the fractions also showed strong hydroxyl radical scavenging activity and nitric oxide scavenging activity as well. Furthermore, cell viability generated by the P. japonicus extract and 4 fractions were examined under C6 glial cellular model. The C6 glial cells showed high generation of reactive oxygen species (ROS) and decrease in cell viability by the treatment generator of hydrogen peroxide. However, the production of ROS formation was decreased by the treatment of the fractions of P. japonicus and also founded that the EtOAc fraction led to significant increase in the cell viability at concentration $100{\mu}g/ml$. Results from this work indicated that P. japonicus showed protective effects against oxidative stress and its EtOAc fraction may be served as a useful natural antioxidant.