율피 추출물이 3T3-L1 지방전구세포 분화에 미치는 영향

• Published in: 동의생리병리학회지 Vol. 24; no. 2; pp. 266 - 271
• Main Author: 이선구(Seon Goo Lee)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2010
• Subjects: 한의학; triglyceride; 3T3-L1 preadipocyte; Chestnut Inner Shell(CIS); Oil Red-O; lipid accumulation; obesity
• ISSN: 1738-7698; 2288-2529; 2283-2529

Obesity occurred by energy imbalance, is increasing regardless of race, sex, age, and related to the metabolic syndrome, diabetes and cardiovascular disease. Since adipose tissue plays a critical role in regulating energy homeostasis, understanding of adipogenesis pathway and finding of regulatory mechanism for adipogenesis can be helpful to manage obesity as well as obesity-related diseases. In this study, to investigate the effects of Chestnut Inner Shell(CIS) extract on the adipogenesis in 3T3-L1 preadipocytes, 3T3-L1 preadipocytes were differentiated with adipogenic reagents for 9 days in the absence or presence of CIS extract ranging from 10 - 100 ${\mu}g/m{\ell}$. The effect of CIS extract on 3T3-L1 differentiation was examined by measuring intracelluar lipid droplet and triglyceride contents. CIS extract remarkably inhibited lipid accumulation(about 45% inhibition at 100 ${\mu}g/m{\ell}$ of CIS extract) and slightly decreased triglyceride contents(about 15% decrease at 100 ${\mu}g/m{\ell}$ of CIS extract) in 3T3-L1 preadipocytes at the concentration showing no cytotoxicity. These results demonstrated that CIS extract significantly inhibit adipogenesis and can be used for the regulation of obesity.