Nicotine으로 유발된 대식세포의 hydrogen peroxide와 Nitric Oxide 생성억제에 대한 효모균발효고삼 추출물의 영향

The effect of Sacchromyces cerevisiae-Fermented Sophorae Radix water extract (SFS) on production of hydrogen peroxide and nitric oxide (NO) from mouse macrophage Raw 264.7 Cells treated with nicotine (1 mM) was investigated through this study. SFS (0, 25, 50, 100, 200, 400 ug/mL) was simultaneously...

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Bibliographic Details
Published in동의생리병리학회지 Vol. 23; no. 5; pp. 1049 - 1054
Main Author 박완수(Wan Su Park)
Format Journal Article
LanguageKorean
Published 한의병리학회 2009
Subjects
한의학
Sophorae Radix
macrophage
fermentation
nitric oxide
Sacchromyces cerevisiae
hydrogen peroxide
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Summary:The effect of Sacchromyces cerevisiae-Fermented Sophorae Radix water extract (SFS) on production of hydrogen peroxide and nitric oxide (NO) from mouse macrophage Raw 264.7 Cells treated with nicotine (1 mM) was investigated through this study. SFS (0, 25, 50, 100, 200, 400 ug/mL) was simultaneously treated with nicotine (1 mM) during culture of 4, 20, 24, 44, 48, 68, and 72 hr. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. NO production after 24 hr treatement was measured with Griess reagent assay. SFS restored the production of hydrogen peroxide and NO reduced by nicotine (1 mM) in Raw 264.7 Cells. These results suggests that SFS could be supposed to have the immunological activity concerned with macrophage's oxidative burst including hydrogen peroxide and NO.
Bibliography:KISTI1.1003/JNL.JAKO200908856862774
G704-000534.2009.23.5.030
ISSN:1738-7698
2288-2529
2283-2529