국내 밀 품종의 글리아딘 및 Spa 유전자 평가

• Published in: Korean journal of breeding Vol. 48; no. 3; pp. 206 - 216
• Main Authors: 김지은(Ji-Eun Kim), 윤영미(Young-Mi Yoon), 조성우(Seong Woo Cho), 강천식(Chon-Sik Kang), 이종열(Jong-Yeol Lee), 박철수(Chul Soo Park)
• Format: Journal Article
• Language: Korean
• Published: 한국육종학회 30.09.2016; The Korean Breeding Society
• Subjects: 농학; A-PAGE; Allelic variation; Storage Protein Activator (Spa); Wheat; Gliadin
• ISSN: 0250-3360; 2287-5174
• DOI: 10.9787/KJBS.2016.48.3.206

Genetic variations of γ-/ω-gliadin and Spa (storage protein activator) in 40 Korean wheat cultivars were evaluated to provide genetic information for improving end-use quality in wheat breeding programs. Korean wheat cultivars were classified into 13 patterns at the Gli-1 locus based on the allelic variation using A-PAGE (acidic-polyacrylamide gel electrophoresis). Seven, five, and six alleles were identified at Gli-A1, Gli-B1, and Gli-D1 loci, respectively. Allele-specific PCR markers for γ-gliadin corresponded to specific allele at Gli-1 loci on A-PAGE, which Gli-A1f, Gli-A1h and Gli-A1l alleles corresponded to GliA1.2, Gli-B1h and Gli-B1f alleles corresponded to GliB1.2 and Gli-D1f, Gli-D1m and Gli-D1o alleles corresponded to GliD1.1. DNA markers for γ-45 and γ-42 also corresponded to the γ-gliadin patterns around 40kDa on A-PAGE, except in Sukang, Ol and Joongmo2003. However, allelic specific PCR markers for ω5-gliadin did not correspond to that of A-PAGE. Three alleles were identified at Spa-A1 locus, whereas there was no variation at Spa-B1 and Spa-D1 loci.