국내 밀 품종의 오메가5-글리아딘 (Gli-B1) 유전자좌 분석

• Published in: Korean journal of breeding Vol. 48; no. 2; pp. 159 - 167
• Main Authors: 장유란(You-Ran Jang), 범혜랑(Hye-Rang Beom), 김정봉(Jung-Bong Kim), 박철수(Chul-Soo Park), 김영태(Yeong-Tae Kim), 강천식(Chon-Sik Kang), 임선형(Sun-Hyung Lim), 김영미(Young-Mi Kim), 이종렬(Jong-Yeol Lee)
• Format: Journal Article
• Language: Korean
• Published: 한국육종학회 30.06.2016; The Korean Breeding Society
• Subjects: 농학; SDS-PAGE; WDEIA; Gli-B1; omega-gliadin
• ISSN: 0250-3360; 2287-5174
• DOI: 10.9787/KJBS.2016.48.2.159

The ω5-gliadins are the major allergens in wheat-dependent excise-induced anaphylaxis (WDEIA). In this study, SDS-PAGE analysis was used to assign the ω5-gliadins (Gli-B1) alleles in thirty two Korean wheat cultivars, compared with eleven standard wheat cultivars for Gli-B1a~m alleles. These results were reconfirmed with their complementary Glu-B3 low-molecular-weight glutenin subunits alleles tightly linked with Gli-B1 locus revealed with 2-DGE in our previous study. As a result, one Gli-B1b, four Gli-B1d, two Gli-B1f, six Gli-B1m and nineteen Gli-B1h varieties were identified. This is the first report on revealing the Gli-B1 alleles in Korean wheat cultivars and represents valuable basic data on wheat allergy, relationship between gliadin and wheat quality, and development of hypo-allergenic wheat.