AZCA 저항성 돌연변이 세포주로부터 선발 육성한 내염성 벼 돌연변이 계통의 특성 검정

• Published in: Journal of plant biotechnology Vol. 34; no. 1; pp. 61 - 68
• Main Authors: 송재영(Jae Young Song), 김동섭(Dong Sub Kim), 이긍주(Geung Joo Lee), 이인석(In Sok Lee), 강권규(Kwon Kyoo Kang), 윤성중(Son
• Format: Journal Article
• Language: Korean
• Published: Korean Society for Plant Biotechnology 31.03.2007; (사)한국식물생명공학회; 한국식물생명공학회
• Subjects: 농학
• ISSN: 1229-2818; 2384-1397

To develop rice (Oryza sativa L.) cultivars to be planted on salt-affected sites, cell lines with enhanced proline content and resistance to growth inhibition by Azetidine-2-carboxylic acid (AZCA), a proline analogue, were screened out among calli irradiated with gamma ray of 50, 70, 90, and 120 Gy. The calli had been derived from embryo culture of the cultivar Donganbyeo. Selected AZCA resistant lines that had high proline accumulation were used as sources for selection of NaCl resistant lines. To determine an optimum concentration for selection of NaCl resistant lines, Donganbyeo seeds were initially cultured on the media containing various NaCl concentrations (0 to 2.5%) for 40 days, and 1.5% NaCl concentration was determined as the optimum concentration. One hundred sixteen salt-tolerant (ST) lines were selected from bulked 20,000 seeds of the AZCA resistant M3 seeds in the medium containing 1.5% NaCl. The putative 33 lines (M4 generation) considered with salt-tolerance were further analyzed for salt tolerance, amino acid and ion contents, and expression patterns of the salt tolerance-related genes. Out of the 33 lines, 7 lines were confirmed to have superior salt tolerance. Based on growth comparison of the entries, the selected mutant lines exhibited greater shoot length with average 1.5 times, root length with 1.3 times, root numbers with 1.1 times, and fresh weight with 1.5 times than control. Proline contents were increased maximum 20%, 100% and 20% in the leaf, seed and callus, respectively, of the selected lines. Compared to control, amino acid contents of the mutants were 24 to 29%, 49 to 143%, 32 to 60% higher in the leaf, seed and callus, respectively. The ratio of Na+/K+ for most of the ST-lines were lower than that of control, ranging from 1.0 to 3.8 for the leaf and 11.5 to 28.5 for the root, while the control had 3.5 and 32.9 in the leaf and root, respectively. The transcription patterns for the P5CS and NHX1 genes observed by RT-PCR analysis indicated that these genes were actively expressed under salt stress. The selected mutants will be useful for the development of rice cultivar resistant to salt stress. KCI Citation Count: 3