털부처꽃의 부위별 성분 분석

• Published in: 大韓本草學會誌 Vol. 37; no. 5; pp. 89 - 96
• Main Authors: 김희영, Hee-young Kim, 박예진, Yea-jin Park, 이주연, Ju-yeon Lee, 김기영, Ki-young Kim, 신수, Su Shin, 최민우, Min-woo Choi, 홍은진, Eun-jin Hong, 김민정, Min-jeong Kim, 여수정, Sujung Yeo, 박인화, In-hwa Park, 정의민, Ui Min Jerng, 안효진, Hyo-jin An, 차윤엽, Yun-yeop Cha
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 30.09.2022
• Subjects: cytotoxicity; DPPH; Lythrum salicaria L; TPC; vitexin; 한의학; Lythrum salicaria L; DPPH; TPC; cytotoxicity; vitexin
• ISSN: 1229-1765; 2288-7199

Objectives : This research was performed to analyze the components in the different parts of Lythrum salicaria L. and to compare which parts of L. salicaria L. are appropriate for food development. Methods : L. salicaria L. was extracted in 20% EtOH at 100 ℃ for 4 hours. Cytotoxicity was investigated in 3T3-L1 cells after treatment of 10-500 μg/ml L. salicaria L. for 24 hours. Total polyphenol content (TPC) was estimated using 1 N Folin-ciocateu reagent. 2,2-Diphenyl-1-picryhydrazyl (DPPH) radical scavenging activity was estimated using DPPH reagent and gallic acid. The chemical composition was analyzed by high-performance liquid chromatography (HPLC). Results : The half maximal inhibitory concentration (IC50) in the extracts of the whole plant, aerial parts, and root parts was 350 μg/ml, over 500 μg/ml, and 150 μg/ml, respectively. The TPC in the extracts of the whole plant, aerial parts, and root parts was 527.1 mg/g, 422.6 mg/g, and 781.1 mg/g, respectively. The averages of vitexin contents in the aerial parts, and root parts were 256.7 ± 154.9 μg/g and 266.1 ± 63.2 μg/g, respectively. The averages of TPC in the leaves, roots, flower stalks and stems were 224.0 ± 53.7 tannin acid (TA) mg/g, 221.8 ± 70.2 TA mg/g, 249.8 ± 34.4 TA mg/g, and 67.7±8.9 TA mg/g, respectively. The averages of DPPH radical scavenging activity in the leaves, roots, flower stalks, and stems were 282.01 ± 43.3 gallic acid equivalent (GAE) μmole/g, 260.16 ± 44.1 GAE μmole/g, 288.0 ± 9.3 GAE μmole/g, and 97.6 ± 10.7 GAE μmole/g, respectively. Conclusions : There were no significant differences in the content of components or antioxidant activity in the aerial parts compared to those in the whole plant of L. salicaria L. Furthermore, the root parts had low extract yield, cytotoxicity, and quality control problems, therefore our results suggest that the use of the aerial part of L. salicaria L. would be the most appropriate for food development.