충남 논산천과 삽교호로부터 야생효모의 분리 및 국내 미기록 효모들의 균학적 특성

• Published in: Hangug gynnhaghoi ji Vol. 49; no. 3; pp. 337 - 349
• Main Authors: 장지은, Ji-eun Jang, 박선정, Seon-jeong Park, 문정수, Jeong-su Moon, 이향범, Hyang Burm Lee, 이종수, Jong-soo Lee
• Format: Journal Article
• Language: Korean
• Published: 한국균학회 30.09.2021
• Subjects: Microbiological characteristics; Nonsan stream; Sapgyoho; Unrecorded yeasts; Wild yeasts; 생물학; Wild yeasts; Microbiological characteristics; Sapgyoho; Nonsan stream; Unrecorded yeasts
• ISSN: 0253-651X; 2383-5249
• DOI: 10.4489/KJM.20210030

This study aimed to isolate wild yeasts from water and soil sample of the Nonsan stream and Sapgyoho (lake) in Chungcheonnam-do, Korea, and to further characterize previously unrecorded wild yeast strains. In total, 102 strains, representing 55 different species of wild yeast were isolated from 95 samples collected from the Jangseoncheon and Ipchoncheon of Nonsan stream in Jellabuk-do and Chungcheonnam-do. Among these, 33 strains were isolated from alkalophilic yeast extract-peptone-dextrose (YPD) medium (pH 9.0), and 9 strains were isolated concurrently on general YPD medium (pH 6.5) and alkalophilic medium. Seventeen strains of Cryptococcus laurentii were predominantly isolated. Additionally, 65 strains, representing 27 different species of wild yeast were isolated from 58 samples obtained from Sapgyoho (lake). Among the 82 isolated wild yeast strains, 8 strains, including Candida fructus JSC 72-1(JSL-GGU 015), had not previously been recorded. All 8 previously unrecorded yeasts were oval in shape except C. fructus JSC72-1(JSL-GGU-015), and only the Filobasidium chernovii JSC39-1(JSL-GGU-013) strain formed spores. All strains except Pseudosydowia eucalypti JSC23-6(JSL-GGU-012) grew well in yeast extract-peptone-dextrose (YPD) and yeast extract-malt extract media and grew in vitamin-free medium. Four strains, including P.eucalypti JSC23-6(JSL-GGU-012) grew well in 15% NaCl-containing YPD medium. F.chernovii JSC39-1(JSL-GGU-013) and Sirobasidium intermedium JSC7-3(JSL-GGU-014) assimilated lactose, and five strains, including F. chernovii JSC39-1(JSL-GGU-013) also assimilated starch. All strains were resistant to 800 ppm of Ca, Cu, Li, and Mg ions.