배초향이 RAW 264.7의 염증인자 생성에 미치는 영향

• Published in: 大韓本草學會誌 Vol. 37; no. 1; pp. 41 - 50
• Main Authors: 박완수, Wansu Park
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 01.01.2022
• Subjects: 7; Agastache rugosa; Cytokine; Lipopolysaccharide; Macrophage; Nitric Oxide; RAW 264; 한의학; Agastache rugosa; 7; Cytokine; Lipopolysaccharide; RAW 264; Nitric Oxide; Macrophage
• ISSN: 1229-1765; 2288-7199

Objectives : The aim of this study was to investigate the effect of water extract of Agastache rugosa (AR) on productions of inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophages. Methods : Cell viabilities were measured with MTT assay. The production of nitric oxide (NO) from RAW 264.7 cells was measured with Griess reagent assay. The production of cytokines in RAW 264.7 cells was measured with multiplex cytokine assay. Results : AR showed no cytotoxicity on RAW 264.7 cells. AR at concentrations of 25, 50, 100, and 200 μg/mL significantly inhibited NO production in LPS-stimulated RAW 264.7 cells. AR at concentrations of 50, 100, and 200 μg/mL significantly inhibited productions of TNF-α and IL-1β in LPS-stimulated RAW 264.7 cells; AR at concentrations of 50 and 200 μg/mL significantly inhibited productions of RANTES (CCL5) in LPS-stimulated RAW 264.7 cells; AR at concentrations of 100 μg/mL significantly inhibited productions of macrophage inflammatory protein-1β in LPS-stimulated RAW 264.7 cells; AR at concentrations of 50, 100, and 200 μg/mL significantly increased productions of IP-10 (CXCL10) in LPS-stimulated RAW 264.7 cells; AR at concentrations of 100 and 200 μg/mL significantly increased MCP-1 (CCL-2) in LPS-stimulated RAW 264.7 cells; AR at concentrations of 50 and 100 μg/mL significantly increased productions of IL-10 in LPS-stimulated RAW 264.7 cells. Conclusions : AR might have immunomodulatory effects on productions of NO, cytokines, and chemokines in LPSstimulated RAW 264.7 mouse macrophages.