HaCaT 세포의 산화 스트레스로 인한 세포자멸사에서 정향의 보호효과

• Published in: 大韓本草學會誌 Vol. 36; no. 5; pp. 93 - 99
• Main Authors: 박숙자, Sook Jahr Park
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 30.09.2021
• Subjects: apoptosis; Caryophylli Flos; caspase; GSH; H 2</ sub>O 2</ sub>; ROS; 한의학; H_2O_2; apoptosis; GSH; Caryophylli Flos; ROS; caspase
• ISSN: 1229-1765; 2288-7199

Objective : Caryophylli Flos has been used in Korean medicine to relieve vomiting and pains caused by chills that make fluid circulation difficult. This study was designed to investigate the protective effect of ethanol extract of Caryophylli Flos (CF) in hydrogen peroxide (H 2 O 2 )-induced apoptotic cell death in human keratinocyte HaCaT cells. Methods : CF was prepared by extracting 200 g of Caryophylli Flos in 2 L of ethanol for 48 h. Cell viability was measured by MTT assay, and the protein expression was monitored by Western blot analysis. Apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Reactive oxygen species (ROS) was measured using fluorescent dye, and reduced glutathione (GSH) was determined with a colorimetric commercial kit. Results : CF protected HaCaT cells from cell death caused by oxidative stress after H 2 O 2 treatment. H 2 O 2 amplified generation of ROS and induced depletion of GSH, whereas these changes in ROS and GSH were inhibited by GF treatment. In addition, H 2 O 2 resulted in apoptosis as assessed by TUNEL assay and the expression of apoptosis regulator proteins. However, cells treated with CF showed a decrease in TUNEL-positive cells and restored the reduced expression of procaspase-9, -3 and PARP. Conclusion : This study showed cytoprotective effects of CF by anti-apoptotic activity while exerting antioxidative activity in H 2 O 2 -treated HaCaT cells. These results suggest that CF could be beneficial in skin damage caused by oxidative stress.