끈적긴뿌리버섯(Oudemansiella mucida)의 최적배양조건 및 배양 추출액의 항균작용에 관한 연구
Oudemansiella mucida, an edible and medicinal mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to produce antifungal substances to inhibit the mycelial growth and spore germination of the plant pathogenic fungi. To produce good amount of antifungal substances from culture med...
Saved in:
| Published in | Hangug gynnhaghoi ji Vol. 39; no. 2; pp. 91 - 98 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | Korean |
| Published |
한국균학회
01.08.2011
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0253-651X 2383-5249 |
Cover
| Abstract | Oudemansiella mucida, an edible and medicinal mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to produce antifungal substances to inhibit the mycelial growth and spore germination of the plant pathogenic fungi. To produce good amount of antifungal substances from culture media, the optimal culture conditions of O. mucida were investigated. The most favorable conditions for the mycelial growth were 25℃ and pH 5 in potato dextrose agar. The most favorable carbon and nitrogen sources promoting mycelial growth were maltose and calcium nitrate, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplemented to the basal medium as a carbon source. The optimal mycelial growth of O. mucida was found in the Hennerberg medium. The crude extract from submerged culture of potato dextrose broth exhibited inhibition of mycelial growth of Colletotrichum acutatum, Botrytis cinerea and Pyricularia oryzae but, fungicidal activity is not good enough to compared with commercially available fungicides tested. Therefore, the antifungal substances extracted from submerged culture of O. mucida might have a potential to be used for biocontrol agent of fungal diseases of plants. |
|---|---|
| AbstractList | Oudemansiella mucida, an edible and medicinal mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to produce antifungal substances to inhibit the mycelial growth and spore germination of the plant pathogenic fungi. To produce good amount of antifungal substances from culture media, the optimal culture conditions of O. mucida were investigated. The most favorable conditions for the mycelial growth were $25^{\circ}C$ and pH 5 in potato dextrose agar. The most favorable carbon and nitrogen sources promoting mycelial growth were maltose and calcium nitrate, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplemented to the basal medium as a carbon source. The optimal mycelial growth of O. mucida was found in the Hennerberg medium. The crude extract from submerged culture of potato dextrose broth exhibited inhibition of mycelial growth of Colletotrichum acutatum, Botrytis cinerea and Pyricularia oryzae but, fungicidal activity is not good enough to compared with commercially available fungicides tested. Therefore, the antifungal substances extracted from submerged culture of O. mucida might have a potential to be used for biocontrol agent of fungal diseases of plants. 본 연구는 식 의약용버섯으로 알려진 끈적긴뿌리버섯(Oudemansiella mucida) 배양액에서 추출한 물질이 항진균효과를 갖고 있는지 규명하기위한 실험을 수행하였다. 균사체의 배양액에서 항진균 물질을 생산하기 위한 전단계로 끈적긴뿌리버섯의 균사생장에 적합한 온도, pH, 탄소영양원, 질소영양원, 탄소/질소 비율 및 최적의 고체배지 등을 선발하기 위한 실험을 수행하였다. 균사생장에 최적인 온도와 pH는 각각 $25^{\circ}C$와 pH 5이었으며, 최적 탄소영양원과 질소영양원은 각각 maltose와 calcium nitrate였다. 균사생장을 위한 탄소/질소 영양원의 비율이 20 : 1이고 배지의 glucose 농도가 3%일 때, 균사생장이 가장 활발하였으며, 최적의 고체배지에는 Hennerberg 배지가 선정되었다. 균사생장의 실험 결과를 토대로 끈적긴뿌리버섯의 균사체를 배양한 후 그 배양여액을 이용하여 식물병원 곰팡이에 대한 항진균 효과를 규명하였다. 끈적긴뿌리버섯의 균사체 배양액에서 추출한 물질과 시판되고 있는 항진균 농약을 이용하여 Colletotrichum acutatum, Botrytis cinerea 및 Pyricularia oryzae 등 식물병원성 곰팡이를 대상으로 균사생장과 포자발아를 저해하는 효과를 조사한 결과 끈적긴뿌리버섯의 균사체에서 생산된 물질은 식물병원균의 균사생장을 저해하는 효과를 나타내었으나 공시된 항진균 농약에 비해 저해효과는 낮게 나타났다. 공시된 식물병원균 포자의 발아를 억제하는 효과는 folpet을 제외한 모든 공시 농약에 비해 낮게 나타났다. 따라서 끈적긴뿌리버섯의 균사체 배양액에서 추출한 물질에는 항균성을 나타내는 물질을 함유되어 있어서 새로운 항진균 제제로 개발할 수 있는 가능성이 있는 것으로 사료된다. Oudemansiella mucida, an edible and medicinal mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to produce antifungal substances to inhibit the mycelial growth and spore germination of the plant pathogenic fungi. To produce good amount of antifungal substances from culture media, the optimal culture conditions of O. mucida were investigated. The most favorable conditions for the mycelial growth were 25℃ and pH 5 in potato dextrose agar. The most favorable carbon and nitrogen sources promoting mycelial growth were maltose and calcium nitrate, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplemented to the basal medium as a carbon source. The optimal mycelial growth of O. mucida was found in the Hennerberg medium. The crude extract from submerged culture of potato dextrose broth exhibited inhibition of mycelial growth of Colletotrichum acutatum, Botrytis cinerea and Pyricularia oryzae but, fungicidal activity is not good enough to compared with commercially available fungicides tested. Therefore, the antifungal substances extracted from submerged culture of O. mucida might have a potential to be used for biocontrol agent of fungal diseases of plants. 본 연구는 식·의약용버섯으로 알려진 끈적긴뿌리버섯 (Oudemansiella mucida) 배양액에서 추출한 물질이 항진균 효과를 갖고 있는지 규명하기위한 실험을 수행하였다. 균사체의 배양액에서 항진균 물질을 생산하기 위한 전단계로 끈적긴뿌리버섯의 균사생장에 적합한 온도, pH, 탄소영양원, 질소영양원, 탄소/질소 비율 및 최적의 고체배지 등을 선발하기 위한 실험을 수행하였다. 균사생장에 최적인 온도와 pH는 각각 25℃와 pH 5이었으며, 최적 탄소영양원과 질소영양원은 각각 maltose와 calcium nitrate였다. 균사생장을 위한 탄소/질소 영양원의 비율이 20 : 1이고 배지의 glucose 농도가 3%일 때, 균사생장이 가장 활발하였으며, 최적의 고체배지에는 Hennerberg 배지가 선정되었다. 균사생장의 실험 결과를 토대로 끈적긴뿌리버섯의 균사체를 배양한 후 그 배양여액을 이용하여 식물병원 곰팡이에 대한 항진균 효과를 규명하였다. 끈적긴뿌리버섯의 균사체 배양액에서 추출한 물질과 시판되고 있는 항진균 농약을 이용하여 Colletotrichum acutatum, Botrytis cinerea 및 Pyricularia oryzae 등 식물병원성 곰팡이를 대상으로 균사생장과 포자발아를 저해하는 효과를 조사한 결과 끈적긴뿌리버섯의 균사체에서 생산된 물질은 식물병원균의 균사생장을 저해하는 효과를 나타내었으나 공시된 항진균 농약에 비해 저해효과는 낮게 나타났다. 공시된 식물병원균 포자의 발아를 억제하는 효과는 folpet을 제외한 모든 공시 농약에 비해 낮게 나타났다. 따라서 끈적긴뿌리버섯의 균사체 배양액에서 추출한 물질에는 항균성을 나타내는 물질을 함유되어 있어서 새로운 항진균 제제로 개발할 수 있는 가능성이 있는 것으로 사료된다. Oudemansiella mucida, an edible and medicinal mushrooms belonging to Tricholomataceae of Basidiomycota,has been known to produce antifungal substances to inhibit the mycelial growth and spore germination of the plant pathogenic fungi. To produce good amount of antifungal substances from culture media, the optimal culture conditions of O. mucida were investigated. The most favorable conditions for the mycelial growth were 25℃ and pH 5 in potato dextrose agar. The most favorable carbon and nitrogen sources promoting mycelial growth were maltose and calcium nitrate, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplemented to the basal medium as a carbon source. The optimal mycelial growth of O. mucida was found in the Hennerberg medium. The crude extract from submerged culture of potato dextrose broth exhibited inhibition of mycelial growth of Colletotrichum acutatum, Botrytis cinerea and Pyricularia oryzae but, fungicidal activity is not good enough to compared with commercially available fungicides tested. Therefore, the antifungal substances extracted from submerged culture of O. mucida might have a potential to be used for biocontrol agent of fungal diseases of plants. KCI Citation Count: 1 |
| Author | Jae Seong Lee Hae Jin Cho 이태수 Tae Soo Lee 조해진 Hye Young Kim 이재성 Mi Ryue Choi 김혜영 최미례 |
| Author_xml | – sequence: 1 fullname: 최미례 – sequence: 2 fullname: Mi Ryue Choi – sequence: 3 fullname: 조해진 – sequence: 4 fullname: Hae Jin Cho – sequence: 5 fullname: 이재성 – sequence: 6 fullname: Jae Seong Lee – sequence: 7 fullname: 김혜영 – sequence: 8 fullname: Hye Young Kim – sequence: 9 fullname: 이태수 – sequence: 10 fullname: Tae Soo Lee |
| BackLink | https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001575163$$DAccess content in National Research Foundation of Korea (NRF) |
| BookMark | eNo9zD1Lw0AAxvEgCtaXT-CSRbBD4C73kstYiu9CQTq4HddeIkfTVBKLuCk4OAgqWBWp0i6CoBilrW5-n-T6HaxWnP7w8OOZMSbDRuhNGDkbMWQRG7uTRg7YBFmUwJ1pYz6OVQUQhBwHMjdnHGTHp7pznH72sq-z7PE5ez_RJ69Lpab06iKMlRcEwqw3q0qKvL6_NXW_PeJZkujrS91N0reemSXn5ngw9eBKD9q61f2hw9ZL-tHRD5f67knfXJhp72jYapv6Jkn7z3PGlC-C2Jv_66xRXlkuF9esrdLqerGwZdUYxpYviQcAw9STPnUhoC6rSFqlGDAmAUTEZxUIfYAYxQJgX2IJnSoWVHgVl0qKZo38-DaMfF6rKt4Q6re7DV6LeGG7vM4hAA7BeGQXx7am4n3FQxkHfKOwWbIBhDa2kUsgcggYuYV_F_O9SNVFdMhtFzkYu-gbkaiQXA |
| ContentType | Journal Article |
| DBID | HZB Q5X JDI ACYCR |
| DEWEY | 579.5 |
| DatabaseName | KISS(한국학술정보) Korean Studies Information Service System (KISS) B-Type KoreaScience Korean Citation Index |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Botany |
| DocumentTitleAlternate | 끈적긴뿌리버섯(Oudemansiella mucida)의 최적배양조건 및 배양 추출액의 항균작용에 관한 연구 The Optimal Culture Conditions and Antifungal Activity of Culture Extract from Oudemansiella mucida |
| EISSN | 2383-5249 |
| EndPage | 98 |
| ExternalDocumentID | oai_kci_go_kr_ARTI_1007544 JAKO201124239513750 2937449 |
| GroupedDBID | 5GY 9ZL ALMA_UNASSIGNED_HOLDINGS GROUPED_DOAJ HZB JDI OK1 Q5X .UV 85H MZR M~E ZZE ACYCR |
| ID | FETCH-LOGICAL-k844-fd5e00846edf6910698bd6c64088d0135f8b11f03864a04fd4d17c4a6aeb96d63 |
| ISSN | 0253-651X |
| IngestDate | Tue Jun 25 21:09:09 EDT 2024 Fri Dec 22 12:03:40 EST 2023 Sat Aug 30 02:20:34 EDT 2025 |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Keywords | Antifugal substance Oudemansiella mucida Culture condition Fungal diseases |
| Language | Korean |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-k844-fd5e00846edf6910698bd6c64088d0135f8b11f03864a04fd4d17c4a6aeb96d63 |
| Notes | The Korean Society of Mycology KISTI1.1003/JNL.JAKO201124239513750 G704-001216.2011.39.2.009 |
| OpenAccessLink | http://click.ndsl.kr/servlet/LinkingDetailView?cn=JAKO201124239513750&dbt=JAKO&org_code=O481&site_code=SS1481&service_code=01 |
| PageCount | 8 |
| ParticipantIDs | nrf_kci_oai_kci_go_kr_ARTI_1007544 kisti_ndsl_JAKO201124239513750 kiss_primary_2937449 |
| PublicationCentury | 2000 |
| PublicationDate | 2011-08 |
| PublicationDateYYYYMMDD | 2011-08-01 |
| PublicationDate_xml | – month: 08 year: 2011 text: 2011-08 |
| PublicationDecade | 2010 |
| PublicationTitle | Hangug gynnhaghoi ji |
| PublicationTitleAlternate | 한국균학회지 |
| PublicationYear | 2011 |
| Publisher | 한국균학회 |
| Publisher_xml | – name: 한국균학회 |
| SSID | ssib053377189 ssib001148631 ssj0000314788 ssib044738303 ssib010782576 |
| Score | 1.767641 |
| Snippet | Oudemansiella mucida, an edible and medicinal mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to produce antifungal substances to... 본 연구는 식·의약용버섯으로 알려진 끈적긴뿌리버섯 (Oudemansiella mucida) 배양액에서 추출한 물질이 항진균 효과를 갖고 있는지 규명하기위한 실험을 수행하였다. 균사체의 배양액에서 항진균 물질을 생산하기 위한 전단계로 끈적긴뿌리버섯의 균사생장에 적합한 온도, pH,... |
| SourceID | nrf kisti kiss |
| SourceType | Open Website Open Access Repository Publisher |
| StartPage | 91 |
| SubjectTerms | Antifugal substance Culture condition Fungal diseases Oudemansiella mucida 생물학 |
| Title | 끈적긴뿌리버섯(Oudemansiella mucida)의 최적배양조건 및 배양 추출액의 항균작용에 관한 연구 |
| URI | https://kiss.kstudy.com/ExternalLink/Ar?key=2937449 http://click.ndsl.kr/servlet/LinkingDetailView?cn=JAKO201124239513750&dbt=JAKO&org_code=O481&site_code=SS1481&service_code=01 https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001575163 |
| Volume | 39 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | 한국균학회지, 2011, 39(2), , pp.91-98 |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Na9RAFB_a4sGLqFWsHyWIA0qIJJvJZOaYpLvUinqp0NuSz-26dlfaLlJPFnrwIGjBWpEq7UUQKq7SVm_-P7vp_-CbSXY39QOqsITHzNs3896bZH5vMpmH0LXQikshMX3NjnioEerDc9CKE81nlCYlIyFRJL53vnOXTt8nM3PW3MjoSmHXUns5uBk--eN3Jf_jVSgDv4qvZP_BswOhUAA0-Beu4GG4HsvHuOwCEsSM4bKHHV3QZQe7DLtEVLkVzDxBOBQ7knBLmBHBDFenIk4abUfxAsxWdbEJSl1oh_UI0CQXLHwKc6YKyrUw9wpNgBxd_AQTxdyQVYYsgdaBIGrOxCrq7-yZTKBIn5DCuSWEFBqekkVTUqgt2hZ15bw97mAn66aNua5KJiK2bWR_456aV-a9AkleEYhPi3XamlpbaTbn_dp8q64-qPcHX0FnV5gJDCoIMLReZMlVlu25UhfHxowUWUCVrAb67Xi54V1jyAI94_1es74hGM7yy_RXY7Ll5cJqzEDJvmpFI2VVXBIODIDCo75kmRq1ZPIgmJVlGWApU7NK2YGu-fSSJTbLgUqWvfvoEeK_TO1HDhFvhPVqrVVtLFYhVLolD6u2CBlFo6ZRXIuQOBqCZDp8PWwIHGkNcSshNnRu-PoeQgYbMA4fLG6KnAi2zPk60AwgD0RESxADisCoDlCuuZgUoNzsaXQqj8EUJ7uhzqCRRussOuG2IE5aGUePe6vP0u3V7ve93o_nvQ-7va9r6drn60fuFCW7U26k794o6f4WsPc6nfT1errT6X7ZU3qdF0pWoKQHr9KDrXRjR7AebnzqfttO36-nbz-mmy-V7t7Tw40tJd3sdPd3z6HZSnnWm9by9CRagxGiJZEVi2wUNI4SCqCbchZENKQE5u0IAisrYYFhJLrJKPF1kkQkMuyQ-NSPA04jap5HY81WM76AlJgHNGB-GIZJQPywFLBYt3yThVyPfEs3JtC4sF31UXYATRUguk0In0CT0pbVZrT0sDrj3L4nRqQIkCBsMiEUmEBXwcjS8X8fABePw3QJnRyO9stobHmxHV8BNL4cTMqR8xPmDcNy |
| linkProvider | Directory of Open Access Journals |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%EB%81%88%EC%A0%81%EA%B8%B4%EB%BF%8C%EB%A6%AC%EB%B2%84%EC%84%AF%28Oudemansiella+mucida%29%EC%9D%98+%EC%B5%9C%EC%A0%81%EB%B0%B0%EC%96%91%EC%A1%B0%EA%B1%B4+%EB%B0%8F+%EB%B0%B0%EC%96%91+%EC%B6%94%EC%B6%9C%EC%95%A1%EC%9D%98+%ED%95%AD%EA%B7%A0%EC%9E%91%EC%9A%A9%EC%97%90+%EA%B4%80%ED%95%9C+%EC%97%B0%EA%B5%AC&rft.jtitle=Hangug+gynnhaghoi+ji&rft.au=%EC%B5%9C%EB%AF%B8%EB%A1%80&rft.au=%EC%A1%B0%ED%95%B4%EC%A7%84&rft.au=%EC%9D%B4%EC%9E%AC%EC%84%B1&rft.au=%EA%B9%80%ED%98%9C%EC%98%81&rft.date=2011-08-01&rft.pub=%ED%95%9C%EA%B5%AD%EA%B7%A0%ED%95%99%ED%9A%8C&rft.issn=0253-651X&rft.eissn=2383-5249&rft.spage=91&rft.epage=98&rft.externalDBID=n%2Fa&rft.externalDocID=oai_kci_go_kr_ARTI_1007544 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0253-651X&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0253-651X&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0253-651X&client=summon |