끈적긴뿌리버섯(Oudemansiella mucida)의 최적배양조건 및 배양 추출액의 항균작용에 관한 연구

• Published in: Hangug gynnhaghoi ji Vol. 39; no. 2; pp. 91 - 98
• Main Authors: 최미례, Mi Ryue Choi, 조해진, Hae Jin Cho, 이재성, Jae Seong Lee, 김혜영, Hye Young Kim, 이태수, Tae Soo Lee
• Format: Journal Article
• Language: Korean
• Published: 한국균학회 01.08.2011
• Subjects: Antifugal substance; Culture condition; Fungal diseases; Oudemansiella mucida; 생물학; Antifugal substance; Oudemansiella mucida; Culture condition; Fungal diseases
• ISSN: 0253-651X; 2383-5249

Oudemansiella mucida, an edible and medicinal mushrooms belonging to Tricholomataceae of Basidiomycota, has been known to produce antifungal substances to inhibit the mycelial growth and spore germination of the plant pathogenic fungi. To produce good amount of antifungal substances from culture media, the optimal culture conditions of O. mucida were investigated. The most favorable conditions for the mycelial growth were 25℃ and pH 5 in potato dextrose agar. The most favorable carbon and nitrogen sources promoting mycelial growth were maltose and calcium nitrate, respectively. The optimum C/N ratio was about 20 : 1 in case that 3% glucose was supplemented to the basal medium as a carbon source. The optimal mycelial growth of O. mucida was found in the Hennerberg medium. The crude extract from submerged culture of potato dextrose broth exhibited inhibition of mycelial growth of Colletotrichum acutatum, Botrytis cinerea and Pyricularia oryzae but, fungicidal activity is not good enough to compared with commercially available fungicides tested. Therefore, the antifungal substances extracted from submerged culture of O. mucida might have a potential to be used for biocontrol agent of fungal diseases of plants.