카드뮴으로 유발한 간세포 자멸사에서 진피의 보호효과

• Published in: 大韓本草學會誌 Vol. 36; no. 1; pp. 41 - 49
• Main Authors: 노규표, Gyu Pyo Noh, 변성희, Sung Hui Byun, 이종록, Jong Rok Lee, 박숙자, Sook Jahr Park, 김상찬, Sang Chan Kim
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 01.01.2021
• Subjects: apoptosis; cadmium; caspase; Citrus unshiu peel; MMP; 한의학; MMP; cadmium; apoptosis; Citrus unshiu peel; caspase
• ISSN: 1229-1765; 2288-7199

Objective : Citrus unshiu peel (Citri Unshius Pericarpium) has been prescribed to suppress coughing and phlegm in Korean medicine. In this study, the effect of ethanol extract of Citrus unshiu peel (CEE) on apoptosis was investigated using cadmium chloride (CdCl2) treated HepG2 cells. Methods : CEE was prepared by extracting 300 g of Citri Unshius Pericarpium in 3 L of ethanol for 72 h. Apoptosis was determined by the TUNEL assay. The mitochondrial membrane potential (MMP) was monitored using the membranepermeable fluorescent dye Rh123. The expression level of each protein was monitored by Western blot analysis. Results : CEE protected HepG2 cells from apoptosis as determined by the TUNEL assay. A decrease in MMP was observed in cells exposed to cadmium, indicating that mitochondria are involved in the induction of apoptosis. However, CEE recovered the reduction in MMP caused by cadmium. In addition, decreased expression of B-cell lymphoma 2 (Bcl-2), procaspase, and poly(ADP-ribose) polymerase (PARP) by cadmium was increased by CEE. The anti-apoptotic effect of CEE was found to be associated with inhibition of JNK and p38 phosphorylation when examining the expression of phosphorylated MAPK by Western blot. Conclusion : This study showed that CEE exerted anti-apoptotic effects in cadmium-induced HepG2 cells by inhibiting the reduction of MMP and changes in the expression level of apoptotic proteins. These results suggest the potential for CEE to be used for heavy metal-induced liver damage.