카드뮴으로 인한 마우스 간 손상에 대한 진피의 보호효과

• Published in: 大韓本草學會誌 Vol. 36; no. 1; pp. 1 - 8
• Main Authors: 노규표, Gyu Pyo Noh, 이종록, Jong Rok Lee, 김재광, Jae Kwang Kim, 박상미, Sang Mi Park, 박숙자, Sook Jahr Park, 김상찬, Sang Chan Kim
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 01.01.2021
• Subjects: 4-hydroxynonenal; cadmium; caspase-3; Citri Unshius Pericarpium; nitrotyrosine; PARP; 한의학; cadmium; nitrotyrosine; PARP; Citri Unshius Pericarpium; 4-hydroxynonenal; caspase-3
• ISSN: 1229-1765; 2288-7199

Objective : Citri Unshius Pericarpium (Citrus unshiu peel) has been used in Korean medicine to treat indigestion, vomiting, coughing and phlegm. This study investigated the hepatoprotective effect of ethanol extract of Citrus unshiu peel (CEE) in cadmium (CdCl2)-treated mouse model. Methods : CEE was dissolved in water and administered orally to mice once a day for 7 consecutive days. The mice were then exposed to a single intraperitoneal (i.p.) injection of cadmium (4 ㎎/㎏ body weight) to induce acute hepatotoxicity. At the end of the experiment, blood and liver tissue samples were collected, analyzed for alanine aminotransferase (ALT), aspartate aminotransferase (AST), and histopathological evaluation. Liver damage was assessed as the percentage of degenerative areas of the hepatic parenchyma, the number of degenerative hepatocytes, and the number of infiltrated inflammatory cells. Results : In cadmium-treated rats, pretreatment with CEE significantly reduced the serum ALT and AST levels associated with liver damage. Histopathologically, CEE prevented degenerative changes on the hepatic tissues including confluent necrosis, congestions and infiltration of inflammatory cells. CEE also reduced the elevation of oxidative stress markers (nitrotyrosine and 4-hydroxynonenal) and apoptosis markers (cleaved caspase-3 and cleaved PARP) positive cells. PARP protein expression in liver tissue was also restored by CEE. Conclusion : This study showed that CEE exerted antioxidant and anti-apoptotic effects against cadmium-induced liver injury. Thus, it can be concluded that CEE can be used to prevent liver damage caused by cadmium.