마가목 줄기 열수 및 에탄올 추출물의 항산화 활성

• Published in: 大韓本草學會誌 Vol. 32; no. 3; pp. 29 - 36
• Main Authors: 유지현, Ji-hyun Yoo, 도은수, Eun-soo Doh, 장준복, Jun-pok Chang, 길기정, Ki-jung Kil
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 31.05.2017
• Subjects: Antioxidant; Ethanol Extracts; Hot-Water Extract; Sorbus commixta; 한의학; Ethanol Extracts; Hot-Water Extract; Antioxidant; Sorbus commixta
• ISSN: 1229-1765; 2288-7199
• DOI: 10.6116/kjh.2017.32.3.29

Objectives : In order to investigate the possibility of Sorbus commixta Heal. stem (SC) as a natural material, antioxidant activities of the hot water and ethanol extracts were examined. Methods : The samples of SC were pulverized, and fractions were extracted repeatedly three times from hot water and 70% ethanol at room temperature for 2 hours. The antioxidant activities were analyzed from total polyphenol, flavonoid contents, DPPH, ABTS, hydroxyl radical, Fe2 + chelating, and nitrite scavenging activity. Results : Total polyphenol contents were significantly higher in ethanol extract group (504.39 ㎍/㎎) than in hot water extract group (364.64 ㎍/㎎). Total flavonoid contents were also significantly higher in ethanol extract group (160.09 ㎍/㎎) than in hot water extract group (124.59 ㎍/㎎). DPPH, ABTS, Fe2 + chelating were slightly higher in ethanol extract gorups than in hot water extract groups, and increased in a dose-dependent manner. The hydroxyl radical scavenging activity (18.42~23.61%) of ethanol extract groups were shown to be approximately twice higher than that (7.63~10.37%) of hot water extract groups at 12.5~50 ㎍/㎖ concentration. Nitrite scavenging activities of both ethanol and hot-water extract groups were shown to be higher in a dose dependent manner at the concentration of 12.5~50 ㎍/㎖ at pH 3.0 than at pH 1.2, and ethanol extract groups (86.55~96.64%) had higher activity than the hot water extract groups (42.59~92.63%), which was higher than that of the control group antioxidant BHT (72.96~80.11%). Conclusions : The extracts of SC displayed antioxidant activities which suggested a natural material can be developed to functional material.