LC-MS/MS를 이용한 농산물 중 Dichlobentiazox 시험법 개발 및 검증

• Published in: Hanguk hwangyeong nonghak hoeji Vol. 40; no. 2; pp. 108 - 117
• Main Authors: 구선영, Sun Young Gu, 이한솔, Han Sol Lee, 박지수, Ji-su Park, 이수정, Su Jung Lee, 신혜선, Hye-sun Shin, 강성은, Sung Eun Kang, 정윤미, Yun Mi Chung, 최하나, Ha Na Choi, 윤상순, Sang Soon Yoon, 정용현, Young-hyun Jung, 윤혜정, Hae Jung Yoon
• Format: Journal Article
• Language: Korean
• Published: 한국환경농학회 30.06.2021
• Subjects: Agricultural Product; Analytical Method; Dichlobentiazox; LC-MS/MS; Pesticide Residue; 농학; Agricultural Product; LC-MS/MS; Analytical Method; Dichlobentiazox; Pesticide Residue
• ISSN: 1225-3537; 2233-4173
• DOI: 10.5338/KJEA.2021.40.2.13

BACKGROUND: Dichlobentiazox is a newly registered pesticide in Korea as a triazole fungicide and requires establishment of an official analysis method for the safety management. Therefore, the aim of this study was to determine the residual analysis method of dichlobentiazox for the five representative agricultural products. METHODS AND RESULTS: Three QuEChERS methods were applied to establish the extraction method, and the EN method was finally selected through the recovery test. In addition, various adsorbent agents were applied to establish the clean-up method. As a result, it was found that the recovery of the tested pesticide was reduced when using the d-SPE method with PSA and GCB, but C18 showed an excellent recovery. Therefore this method was established as the final analysis method. For the analysis, LC-MS/MS was used with consideration of the selectivity and sensitivity of the target pesticide and was operated in MRM mode. The results of the recovery test using the established analysis method and inter laboratory validation showed a valid range of 70-120%, with standard deviation and coefficient of variation of less than 3.0% and 11.6%, respectively. CONCLUSION: Dichlobentiazox could be analyzed with a modified QuEChERS method, and the method determined would be widely available to ensure the safety of residual pesticides in Korea.