가래나무 수피의 페놀성 화합물

Juglans mandshurica barks were collected, extracted with acetone-$H_2O$ (7:3, v/v), fractionated with n -hexane, $CH_2Cl_2$, and EtOAc, and freeze dried to give some dark brown powder. The EtOA cand $H_2O$ soluble fractions were chromatographe d on a Sephadex LH-20 column using $H_2O$-MeOH and EtOH-...

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Bibliographic Details
Published in목재공학 Vol. 34; no. 6; pp. 51 - 60
Main Authors 김진규, Jin Kyu Kim, 사전령, Chuan Ling Si, 배영수, Young Soo Bae
Format Journal Article
LanguageKorean
Published 한국목재공학회 30.11.2006
Subjects
flavonoid glycosides
Juglans mandshurica
NMR
Phenolic compounds
Sephadx LH-20
임학
phenolic compounds
NMR
Juglans mandshurica
flavonoid glycosides
Sephadex LH-20
Online AccessGet full text
ISSN1017-0715
2233-7180

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Summary:Juglans mandshurica barks were collected, extracted with acetone-$H_2O$ (7:3, v/v), fractionated with n -hexane, $CH_2Cl_2$, and EtOAc, and freeze dried to give some dark brown powder. The EtOA cand $H_2O$ soluble fractions were chromatographe d on a Sephadex LH-20 column using $H_2O$-MeOH and EtOH-hexane mixture as eluents. Spectrometric analysis such as NMR and MS, including TLC,were performed to characterize the structures of the isolated compounds. From the EtOAc and $H_2O$ soluble fractions, three flavanols (1~3), three flavonols (4~6) and five flavonol glycosides (7~11) were isolated and elucidated. 가래나무 수피를 채취하여 건조시킨 후 분말로 제조하고 3.2 kg을 아세톤-물(7:3, v/v) 혼합용액으로 추출하여 유기용매를 제거한 후 헥산, 메틸렌클로라이드, 에틸아세테이트 및 물을 사용하여 네 개로 분획하고 에틸아세테이트 및 수용성 분획물에 대하여 칼럼 크로마토그래피를 수행하였으며 용리용매로는 메탄올 수용액과 에탄올-헥산 혼합액을 사용하였다. 그 결과 flavanol 화합물인 pinobanksin, taxifolin 및 ampelopsin, flavonol 화합물인 kaempferol, quercetin 및 myricetin 과 flavone glycoside 화합물인 afzelin, astragalin, quercitrin, isoquercitrin 및 myricitrin을 단리하였으며 NMR 및 MS 스펙트럼을 이용하여 구조를 결정하였다.
Bibliography:The Korean Society of Wood Science & Technology
KISTI1.1003/JNL.JAKO200610103463207
G704-000240.2006.34.6.006
ISSN:1017-0715
2233-7180