인간 말초혈액 단핵구 유래 수지상세포의 면역반응에 미치는 Gefitinib의 영향

• Published in: Tuberculosis and respiratory diseases Vol. 69; no. 6; pp. 456 - 464
• Main Authors: 김미현, Mi Hyun Kim, 김기욱, Ki Uk Kim, 박혜경, Hye Kyung Park, 이민기, Min Ki Lee, 김윤성, Yun Seong Kim, 박순규, Soon Kew Park, 전두수, Doo Soo Jeon, 조진훈, Jin Hoon Cho, 이광하, Kwang Ha Lee
• Format: Journal Article
• Language: Korean
• Published: 대한결핵 및 호흡기학회 30.12.2010; 대한결핵및호흡기학회
• Subjects: Apoptosis; Dendritic cells; gefitinib; Interleukin-12; 내과학; gefitinib; Dendritic cells; Interleukin-12; Apoptosis
• ISSN: 1738-3536; 2005-6184

Background: Synergistic antitumor effects of the combined chemoimmunotherapy based on dendritic cells have been reported recently. The aim of this study is to search new applicability of gefitinib into the combination treatment through the confirmation of gefitinib effects on the monocyte derived dendritic cells (moDCs); most potent antigen presenting cell (APC). Methods: Immature and mature monocyte-derived dendritic cell (im, mMoDC)s were generated from peripheral blood monocyte (PBMC) in Opti-MEM culture medium supplemented with IL-4, GM-CSF and cocktail, consisting of TNF-α (10 ng/mL), IL-1β (10 ng/mL), IL-6 (1,000 U/mL) and PGE(2) (1 μ/mL). Various concentrations of gefitinib also added on day 6 to see the influence on immature and mature MoDCs. Immunophenotyping of DCs under the gefitinib was performed by using monoclonal antibodies (CD14, CD80, CD83, CD86, HLA-ABC, HLA-DR). Supernatant IL-12 production and apoptosis of DCs was evaluated. And MLR assay with [3H]-thymidine uptake assay was done. Results: Expression of CD83, MHC I were decreased in mMoDCs and MHC I was decreased in imMoDCs under gefitinib. IL-12 production from mMoDCs was decreased under 10 μM of gefitinib sinificantly. Differences of cell proliferation capacity were not observed in each concentration of geftinib. Conclusion: In spite of decreased expressions of some dendritic cell surface molecules and IL-12 production under 10 μM of gefitinib, significant negative influences of gefitinib in antigen presenting capacity and T cell stimulation were not observed.