Differentiation of Actinomycete Genera Based on Partial rpoB Gene Sequences

• Published in: Journal of microbiology and biotechnology Vol. 13; no. 6; pp. 846 - 852
• Main Authors: Kim, Bum-Joon, Koh, Young-Hwan, Chun, Jong-Sik, Kim, Chang-Jin, Lee, Seung-Hyun, Cho, Moon-Jae, Hyun, Jin-Won, Lee, Keun-Hwa, Cha, Chang-Yong, Kook, Yoon-Hoh
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 30.12.2003; 한국미생물·생명공학회
• Subjects: actinomycete genera; identification; Phylogenetic relationship; rpoB; signature amino acids. differentiation; 생물학; Phylogenetic relationship; signature amino acids; differentiation; identification; rpoB; actinomycete genera
• ISSN: 1017-7825; 1738-8872

rpoB DNAs (279 bp) from 34 species of 5 actinomycete genera were sequenced and a phylogenetic tree was constructed based on the sequences obtained. The genera were clearly differentiated in the rpoB tree, forming clades specific to their respective genus. In addition, 2 signature amino acid residues specific to Streptomyces were found in a multiple alignment of the deduced amino acid sequences. To empirically confirm that this rpoB gene analysis system could be used to differentiate actinomycete isolates, the proposed system was used to identify 16 actinomycete isolates from Jeju Island. All isolates were successfully differentiated into the genera Streptomyces and Micromonospora. Accordingly, this is the first report that an rpoB sequence analysis has been effectively used to differentiate actinomycete strains at the genus level.