Isolation of Streptomyces sp. YU100 Producing Extracellular Phospholipase D

• Published in: Journal of microbiology and biotechnology Vol. 12; no. 1; pp. 71 - 76
• Main Authors: Lim, Si-Kyu, Choi, Jae-Woong, Lee, Eun-Tag, Khang, Yong-Ho, Kim, Sang-Dal
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 01.01.2002; 한국미생물·생명공학회
• Subjects: 생물학; phylogeny; 16S rDNA; transphosphatidylation; Streptomyces sp; Phospholipase D
• ISSN: 1017-7825; 1738-8872

Soil samples were screened for actinomycete strains capable of producing phospholipase D, and a strain, Streptomyces sp. YU100, showing a high transphosphatidylation activity was isolated. This strain secreted phospholipase D in a culture broth after 12 h of cultivation, and its productivity continued to increase for 36 h of fermentation. In addition, its transphosphatidylation rate of phosphatidylcholine to phosphatidylserine was almost $68\%$ within 1 h. The morphological and chemotaxonomical characteristics showed that this strain could be classified as a number of the Streptomycetaceae family, particularly due to the spiral form of its spore chain consisting of 60-70 smooth spores $(0.75{\times}1.0{\mu}m$) on an aerial mycelium, FA-2c type of fatty acid profile in the cell wall, and LL-DAP component in the cell wall peptidoglycan. A phylogenetic analysis of the 16S rDNA provided a clue that the strain YU100 was actually a member of the genus Streptomyces, because the determined sequence exhibited a higher homology with Streptomyes sp. ASB27, S. peucetius JCM9920, and S. griseus ATCC10137. A dendrogram based on the 16S rDNA sequences also showed a phylogenetic relationship between the strain YU100 and these strains. However, the strain YU100 has not yet been assigned to a particular species, because of absence of any other classified species with a high matching score.