GC-ECD를 이용한 농산물 중 제초제 aclonifen의 공정분석법 확립

• Published in: Hanguk hwangyeong nonghak hoeji Vol. 33; no. 4; pp. 388 - 394
• Main Authors: 고아영, Ah Young Ko, 김희정, Hee Jung Kim, 장진, Jin Jang, 이은향, Eun Hyang Lee, 주윤지, Yoon Ji Joo, 권찬혁, Chan Hyeok Kwon, 손영욱, Young Wook Son, 장문익, Moon Ik Chang, 이규식, Gyu Seek Rhee
• Format: Journal Article
• Language: Korean
• Published: 한국환경농학회 31.12.2014
• Subjects: Aclonifen; GC-ECD; Herbicide; Maximum residue limits; 농학; GC-ECD; Aclonifen; Maximum residue limits; Herbicide
• ISSN: 1225-3537; 2233-4173
• DOI: 10.5338/KJEA.2014.33.4.388

BACKGROUND: Aclonifen is used as a systemic and selective herbicide to control a wide spectrum broad-leaf weeds by inhibition carotenoid biosynthesis, and then its MRLs(Maximum Residue Limits) will be determined in onion and garlic. In this study, a new official method was developed for aclonifen determination in agricultural products to routinely inspect the violation of MRL as well as to evaluate the terminal residue level. METHODS AND RESULTS: Aclonifen was extracted from crop samples with acetone and the extract was partitioned with dichloromethane and then purified by silica solid phase extraction(SPE) cartridge. The purified samples were detected GC using an ECD detector. Limits of detection(LOD) was 0.001 mg/kg and quantification(LOQ) was 0.005 mg/kg, respectively. For validation purposes, recovery studies were carried out at three different concentration levels (LOQ, 10×LOQ, 50×LOQ, n=5). Therecoveries were ranged from 74.3 to 95.0% with relative standard deviations(RSDs) of less than 8%. All values were consistent with the criteria ranges requested in the Codex guidelines(CAC/GL 40). CONCLUSION: The proposed analytical method was accurate, effective and sensitive for aclonifen determination and it will be used to as an official method in Korea.