Production of Cyclodextrins in Ultrafiltration Membrane Reactor Containing Cyclodextrin Glycosyltransferase from Bacillus macerans

• Published in: Journal of microbiology and biotechnology Vol. 18; no. 4; pp. 725 - 729
• Main Authors: Son, Young-Jin, Rha, Chan-Su, Park, Yong-Cheol, Shin, So-Yeon, Lee, Yoon-Seung, Seo, Jin-Ho
• Format: Journal Article
• Language: Korean
• Published: 한국미생물생명공학회 30.04.2008; 한국미생물·생명공학회
• Subjects: a-cyclodextrin; Cycldextrin glycosyltransferase; enzyme reactor; immobilization; ultrafiltration membrane; 생물학; enzyme reactor ${\alpha}$-cyclodextrin; immobilization; Cyciodextrin glycosyltransferase; ultrafiltration membrane
• ISSN: 1017-7825; 1738-8872

An enzyme reactor installed with ultrafiltration membrane was developed to produce ${\alpha}-,\;{\beta}-$, and ${\gamma}$-cyclodextrins (CDs) from soluble starch by Bacillus macerans cyclodextrin glycosyltransferase (CGTase) tagged with 10 lysines at its C-terminus (CGTKIOase). Ultrafiltration membrane YM10 with 10,000 of molecular cutoff was chosen for membrane modification and CD production. A repeated-batch type of the enzyme reaction with free CGTK10ase resulted in a ${\alpha}$-CD yield of 24.0 (${\pm}1.5$)% and a productivity of 4.68 (${\pm}0.88$) g/l-h, which were 7 times higher that those for CGTK10ase immobilized on modified YM10 membrane. Addition of 1-nonanol increased CD yields by 30% relative to the control, which might be due to prevention of the reversible hydrolysis of CDs.