보허탕가감 투여가 산후 생쥐의 유즙분비량 및 유즙분비 관련 인자에 미치는 영향

• Published in: 大韓韓方婦人科學會誌 Vol. 29; no. 1; pp. 35 - 52
• Main Authors: 이아영, Ah Yeong Lee, 이은희, Eun Hee Lee, 임지영, Ji Yeong Im, 김홍준, Hong Jun Kim, 이창현, Chang Hyun Lee
• Format: Journal Article
• Language: Korean
• Published: 대한한방부인과학회 25.02.2016
• Subjects: AQP; aquaporin; Boheotang; galactagogues; lactation; 한의학; Boheotang; aquaporin (AQP); lactation; galactagogues
• ISSN: 1229-4292; 2508-3619
• DOI: 10.15204/jkobgy.2016.29.1.035

Objectives: The purpose of this study was to investigate the effect on factors related the expression of aquaporins (AQP) and milk production after administration of Boheotang-gagam in lactating mice. Methods: The SKH-1 mice were randomly allocated to the control group which was administered with distilled water for two weeks after the parturition and the experimental groups such as, lactating+400G group (L400G) which was administered with Boheotang-gagam 400 mg/day, lactating+600G group (L600G) which was administered with 600 mg/day for two weeks after the parturition, and 400G+lactating+400G group (400G-L400G) which was administered with 400 mg/day for 3 weeks starting one week prior to parturition for experiment (n=6 per group). Results: 1. With regard to the immunohistochemical staining reaction for AQP1, AQP3, and AQP5, stronger immune response was also showed in mammary gland in all experimental groups as compared to the control group. AQP1 showed stronger immune response in the capillaries and venules which were located around the interlobular duct, while stronger immune response of AQP3 and AQP5 showed in the secretory alveolar epithelia and intralobular and interlobular ductal epithelial cells. 2. In the western blot, L400G group showed the most increased expression followed by L600G and then 400G-L400G group in AQP1. In AQP3, the order of expression density was observed as L600G, 400G-L400G and L400G group. Lastly, in AQP5, L400G group presented the most increased expression followed by L600G and 400G-L400G group. Conclusions: Boheotang-gagam would have the effect of increasing the lactation of mice after the birth by increasing the prolactin level and adjusting the expressionof AQPs and prolactin receptor in the mammary glands.