가미옥병풍산(加味玉屛風散)의 비만세포에서의 알레르기 반응에 대한 효과 연구

• Published in: 大韓本草學會誌 Vol. 26; no. 4; pp. 1 - 7
• Main Authors: 정진기, Jin Ki Jung, 박용기, Yong Ki Park
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 30.12.2011
• Subjects: anti-allergic effect; modified-Okbyungpoongsan; RBL-2H3 mast cell; 한의학; RBL-2H3 mast cell; modified-Okbyungpoongsan; anti-allergic effect
• ISSN: 1229-1765; 2288-7199

Objectives: In this study, we investigated the effect of modified-Okbyungpoongsan (mOP) on mast cell-mediated allergic response in basophilic leukemia cell line, RBL-2H3 mast cells. Methods: Cells were stimulated with anti-DNP-IgE after the treatment of DNP-HSA (AI/D), and then incubated with different concentrations of mOP (0.1, 0.2, 0.5 and 1 ㎎/㎖) in RBL-2H3 cells. Cell toxicity was determined by WST-1 assay. The degranulation of mast cells was observed by microscope with toluidine blue staining and also the levels of beta-hexosaminidase, histamine and TNF-alpha were measured in culture supernatants by enzyme-linked immunosorbant assay. Results: mOP inhibited anti-DNP-IgE-imduced degranulation of mast cells in RBL-2H3 cells. mOP also significantly decreased the levels of histamine and inflammatory cytokine, TNF-alpha in RBL-2H3 cells, but slightly decreased the level of beta-hexosaminidase. Conclusions: These results indicate that mOP, an oriental prescription could be inhibit the allergic response through suppressing the mast cell activation.