구강암 세포주에서 김 추출물에 의한 세포자멸사 유도

• Published in: 大韓本草學會誌 Vol. 30; no. 2; pp. 25 - 30
• Main Authors: 김상찬, Sang Chan Kim, 이종록, Jong Rok Lee, 박숙자, Sook Jahr Park
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 31.03.2015
• Subjects: apoptosis; oral cancer cells; Porphyra tenera; 한의학; Porphyra tenera; apoptosis; oral cancer cells
• ISSN: 1229-1765; 2288-7199

Objectives : Laver (Porphyra tenera), a red algae species, is one of the most widely consumed edible seaweed in Korea. Laver contains various substances such as essential amino acid, fiber, minerals and polyphenols that benefit human health. In the present study, we prepared ethanol extracts from commercially processed product of Porphyra tenera, and evaluated the growth inhibitory effect against human oral squamous carcinoma YD-10B cells. Methods : Cell viability was measured by MTT assay. Apoptosis was confirmed by TUNEL assay and flow cytometry with the green fluorescent dye FITC annexin V entering apoptotic cells and the red fluorescent dye PI not entering. The expression of the relevant proteins was detected using Western blot. Results : Ethanol extracts of Porphyra tenera (PTE, 50-200 ㎍/㎖) caused a significant decrease of cell viability in a dose dependant manner. The cell death occurred as a result of apoptotic process as determined by TUNEL assay and flow cytometric analysis. In line with this observation, decrease in procaspase proteins and increase in cytosolic cytochrome c were observed in cells treated with PTE. In addition, exposure to PTE decreased the expression levels of Bcl-2, and induced PARP cleavage and AIF translocation from mitochondria to nucleus. Conclusions : In conclusion, PTE exerts anti-cancer effects by inducing apoptosis via caspase activation and AIF nuclear translocation in YD-10B cells. These results provide evidence for the possible therapeutic effect of Porphyra tenera in oral cancer cells.