생쥐의 배양 대뇌신경세포에 대한 Hydrogen Peroxide의 세포독성 및 천마의 영향

• Published in: 동의생리병리학회지 Vol. 16; no. 5; pp. 928 - 931
• Main Authors: 최유선(Yu Sun Choi), 이은미(Eun Mi Lee), 손영우(Young Woo Son), 이강창(Kang Chang Lee), 신용일(Yong Il Shin), 송명수(Myung Su Song), 최영자(Young Ja Choi), 최규철(Kyu Chul Choi), 강형원(Hyung Won Kang), 임창용(Chang Yong Lim), 류지용(Ti Yong Rhu), 박세홍(Sea Hong Park), 박승택(Seung Taeck Park)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2002
• Subjects: Rhizoma Gastrodiae; Oxidative stress; Hydrogen peroxide; Cerebral neuron
• ISSN: 1738-7698; 2288-2529; 2283-2529

To elucidate the toxic effect of oxygen free radicals on cultured mouse cerebral neurons damaged by hydrogen peroxide(H₂O₂)-induced neurotoxicity, we examined the neurotoxicity induced by oxygen radicals by NR assay when cultured cerebral neurons were grown in the media containing various concentrations of H202 for 6 hours. In addition, neuroprotective effects of herb extracts such Rhizoma Gastrodiae(RG) on H202-induced neurotoxicity in cultured cerebral neurons were evaluated after cultured cerebral neurons were preincubated with various concentrations of herb extract, RG for 2 hours before 50uM H₂O₂ for 6 hours. H₂O₂ decreased remarkably cell viability in dose-and time-dependent manner in these cultures, and also herb exract, RG decreased LDH activity of cerebral neurons damaged by H₂O₂. From the above results, it is suggested that H₂O₂ was toxic in cultured cerebral neurons from mouse, and RG was effective in blocking the neurotoxicity induced by oxygen radicals in these cultures.