관절염 모델에서 백굴채전탕액이 척수와 척수신경절의 calcitonin gene-related peptide와 substance P 면역반응에 미치는 영향

• Published in: 동의생리병리학회지 Vol. 16; no. 2; pp. 272 - 278
• Main Authors: 박종주(Jong Joo Park), 육태한(Tae Han Yook), 송범용(Beom Yong Song), 이광규(Kwang Gyu Lee), 유윤조(Yun Jo Yu), 이창현(Chang Hyun Lee)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 2002
• Subjects: Arthritis; Spinal Cord; Substance P; Herba Chelidonii extracts; CGRP
• ISSN: 1738-7698; 2288-2529; 2283-2529

To investigate the anti-inflammatory and analgesic effects of Herba Chelidoniie, the extracts of Herba Chelidoniie treated in arthritic rat model. Complete Freund,s Adjuvant(CFA) were injected in the subcutaneous tissue of left foot paw of rats to induce arthritis. Herba Chelidonii extracts(HC) was administered immediately into the peritoneal cavity after CFA injection for 12 days. The immunohistochemical stainings for calcitonin gene-related peptide(CGRP) and substance P in the L4, L5 and L6 spinal dorsal horn and ganglia were done, and the paw swelling was measured with a micrometer and the blood leukocytes were counted. The results were as follows : The paw swelling of HC treated group was significantly decreased in 12th day after CFA injection compare to control group. The change of differential leukocytes counts of HC treated group increased the ratio of lymphocytes, and decreased the ratio of neutrophils compare to control group. The extent of CGRP immunoreactive nerve fiber of dorsal horn of HC treated group was weakly stained compare to control group. The number of CGRP immunoreactive neurons of L6 spinal cord of HC treated group was significantly decreased compare to control group. The extent of substance P immunoreactive nerve fiber of dorsal horn of He treated group was weakly stained compare to control group. The number of substance P immunoreactive neurons of L4, L5 and L6 spinal cord of HC treated group was significantly decreased compare to control group. These experimental results suggest that Herba Chelidonii extracts reduce the number of CGRP and substance P immunoreactive neurons and nerve fibers of spinal dorsal horns and ganglia, and decrease paw swelling in arthritic rat model, which may be closely related to analgesic and antiinflammatory effects of Herba Chelidonii.