연잎, 연꽃, 연꽃 수술 추출물이 UVB 자외선 조사에 의한 각질형성세포의 보호 및 피부 노화 방지에 미치는 영향

• Published in: 大韓本草學會誌 Vol. 26; no. 2; pp. 45 - 49
• Main Authors: 장문석, Mun Seog Chang, 고은빛, Eun Bit Ko, 이호진, Ho Jin Lee, 김주성, Ju Sung Kim, 김진수, Jin Soo Kim, 지성원, Sung Won Jee, 김휴영, Hyu Young Kim, 염명훈, Myeong Hoon Yeom, 김덕희, Duck Hee Kim, 김한곤, Han Kon Kim, 박성규, Seong Kyu Park
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 30.06.2011
• Subjects: HaCaT keratinocyte; Nelumbo nucifera; Ultraviolet-B; UVB; 한의학; Ultraviolet-B (UVB); Nelumbo nucifera; HaCaT keratinocyte
• ISSN: 1229-1765; 2288-7199

Objectives: The purpose of this study was to investigate the anti-aging effects on cultured human keratinocytes with Nelumbo nucifera extracts. Methods: Each parts of leaves, flowers and stamen were extracted with water or 70% ethanol. These extracts were tested for cell viability on HaCaT cells (human keratinocyte line) by MTT assay. We investigated the effects of Ultraviolet-B (UVB) irradiation on cytotoxicity and lipid peroxidation in cultured skin keratinocytes. Results: The ethanol extract of Nelumbo nucifera flowers showed maximun cell viability as 111.39% in 30 ug/ml concentration. The water extracts of stamen, flowers, leaves showed cell viability as 107.12, 101.65, 101.46%, respectively. HaCaT keratinocytes were survived 63.06% at 20 mJ/cm2 UVB irradiation. The cell membrane lipid peroxidation was measured by accumulation malondialdehyde (MDA). The levels of MDA were decreased by the ethanol extract of Nelumbo nucifera flowers and the water extracts of stamen. Conclusions: These finding suggest that the ethanol extract of Nelumbo nucifera flowers prevent anti-aging effects on cultured human keratinocytes during UVB irradiation.