오령지 추출물의 염증성 세포활성물질 억제효과

• Published in: 大韓本草學會誌 Vol. 24; no. 3; pp. 153 - 160
• Main Authors: 김병진, Byung Jin Kim, 함경완, Kyung Wan Ham, 박경배, Kyung Bae Park, 김대현, Dae Hyeon Kim, 조범연, Beom Yeon Jo, 조창래, Chang Re Cho, 조길환, Gil Hwan Cho, 배기상, Gi Sang Bae, 박경철, Kyoung Chel Park, 구본순, Bon Soon Koo, 김민선, Min Sun Kim
• Format: Journal Article
• Language: Korean
• Published: 대한본초학회 30.09.2009
• Subjects: inflammation. RAW 264.7 cells. Endotoxin shock; Lipopolysaccharide; LPS; TF; Trogopterorum Faeces; 한의학; Trogopterorum Faeces (TF); lipopolysaccharide (LPS); RAW 264; 7 cells; Endotoxin shock; inflammation
• ISSN: 1229-1765; 2288-7199

Objectives: The purpose of this study was to investigate the anti-inflammatory effects of extract from Trogopterorum Faeces (TF) on the RAW 264.7 cells. Methods: To prove the TF`s anti-inflammatory effects, we investigated nitric oxide (NO) production and own cell viability. We examined the cytokine productions on lipopolysacchride (LPS)-induced RAW 264.7 cells and also cellular regulatory mechanisms. Results: TF does not have any cytotoxic effect. TF reduced LPS-induced NO production, interleukin (IL)-1b, IL-6, IL-10 and tumor necrosis factor-a (TNF-a) in RAW 264.7 cells. TF inhibited the activation of mitogen-activated protein kinases (MAPKs) such as p38, extracelluar signal-regulated kinase (ERK 1/2) and c-Jun NH2-terminal kinase (JNK) and also the degradation of inhibitory kappa B a (Ik-Ba) in the LPS-stimulated RAW 264.7 cells. TF reduced the serum levels of IL-1b, IL-6, TNF-a. The survival rate of LPS-induced endotoxin shock was increased by TF administration. Conclusions: TF down-regulated LPS-induced NO and cytokines production, which could provide a clinical basis for anti-inflammatory properties.