설사환자에서 분리한 장출혈성 대장균과 장병원성 대장균의 Enterocyte Effacement 관련 LEE 유전자의 병원형 특성

• Published in: Journal of bacteriology and virology Vol. 37; no. 2; pp. 69 - 78
• Main Authors: 박도훈, Do Hun Park, 문지영, Ji Young Moon, 김영부, Yung Bu Kim
• Format: Journal Article
• Language: Korean
• Published: 대한바이러스학회 2007; 대한미생물학회
• Subjects: AEEC; Attaching and effacing; LEE; Multiplex PCR; Multiplex PCR; Attaching and effacing; AEEC; LEE
• ISSN: 1598-2467; 2093-0429

Attaching and effacing Escherichia coli (AEEC) cause enteric infections in humans and animals. Attaching indicates the intimate attachment of bacteria to the enterocyte, and effacing relates to the localized effacement of brush border microvilli. Enteropathogenic (EPEC) and enterohemorrhagic Escherichia coli (EHEC) infections are characterized by the formation of attaching and effacing (AE) lesion on the intestinal epithelial cells. Therefore, they are often grouped together as AEEC. Development of multiplex PCR allowed us to type five of the most important genes implicated in the formation of the AE lesion. A total of 60 AEEC strains isolated from diarrheal patients were investigated by multiplex PCR for the presence of the insertion site of locus of enterocyte effacement (LEE) and LEE-related (eae, tir, espA, espB, and espD) genes. Associating the results of LEE genes typing in the AEEC strains, three different pathotypes are determined: $eae_{\gamma}-tir_{\gamma}-espA_{\gamma}-espB_{\gamma}-espD_{\gamma}$ (O157:H7), $eae_{\beta}-tir_{\beta}-espA_{\beta}-espB_{\beta}-espD_{\beta}$ (O26:H11), and $eae_{\alpha}-tir_{\alpha}-espA_{\alpha}-espB_{\alpha}-espD_{\alpha}$ (O55:H6). These results indicate that AEEC are a heterogenous groups of organisms.