만성 허혈 후 통증모델에서 발생한 건측의 이질통과 척수의 중추감작

• Published in: Korean journal of anesthesiology Vol. 56; no. 4; pp. 419 - 424
• Main Authors: 곽경화, Kyung Hwa Kwak, 정경영, Kyung Young Jung, 최지영, Jy Young Choi, 류태하, Tae Ha Ryu, 여진석, Jin Seok Yeo, 박성식, Sung Sik Park, 임동건, Dong Gun Lim, 김시오, Si Oh Kim, 백운이, Woon Yi Baek, 홍정길, Jung Gil Hong
• Format: Journal Article
• Language: Korean
• Published: 대한마취통증의학회(구 대한마취과학회) 30.04.2009; 대한마취통증의학회
• Subjects: Chronic post-ischemia pain; Complex regional pain syndrome-type 1; Mirror-image allodynia; NMDA receptor; 마취과학
• ISSN: 2005-6419; 2005-7563

Background: Mirror-image allodynia is a mysterious phenomenon that occurs in association with many clinical pain syndromes including complex regional pain syndromes (CRPS). Underlying mechanisms for the development of such pain are still a matter of investigation. Several studies suggest that activation of the N-methyl-D-aspartate (NMDA) receptor is essential for central sensitization as a base for persistent pain. The aim is to assess whether alteration of NMDA receptor expression correlates with the contralateral allodynia in the chronic post-ischemia pain (CPIP) model rats representing CRPS-Type I. Methods: Application of a tight-fitting tourniquet for a period of 3 hours before reperfusion produced CPIP in male Sprague-Dawley rats. The mechanical paw withdrawal thresholds to von Frey stimuli (using a dynamic plantar aesthesiometer) were measured as pain indicators in ipsilateral and contralateral hindpaws. Phosphorylation of the NMDA receptor 1 subunit (pNR1), assessed with Western blot, was measured in the contralateral L4-6 spinal cord. Results: Ipsilateral and contralateral mechanical allodynia is present at 4 hours after reperfusion, peaked at 3 days, and continued for 7 days after reperfusion. The relative density of pNR1 of CPIP rats significantly decreased in the contralateral L4-6 spinal cord compared to baseline value (P＜0.05). There was significant correlation between paw withdrawal threshold and the relative density of pNR1 (ipsilateral; R2=0.75, P＜0.01, contralateral; R2=0.60, P＜0.01). Conclusions: These data suggest that pNR1 is correlated to the contralateral mechanical allodynia in CPIP rats. (Korean J Anesthesiol 2009;56:419~24)