신생 백서의 저산소-허혈 내성 모델에서 미토콘드리아 ATP 민감성 포타슘 채널 길항제가 전조건화에 미치는 영향

• Published in: Korean journal of anesthesiology Vol. 57; no. 6; pp. 729 - 736
• Main Authors: 박영수, Young Soo Park, 황보영, Bo Young Hwang, 박평환, Pyung Hwan Park, 방지연, Ji Yeon Bang, 정성문, Sung Moon Jeong, 류해영, Hae Young Ryu
• Format: Journal Article
• Language: Korean
• Published: 대한마취통증의학회(구 대한마취과학회) 30.12.2009; 대한마취통증의학회
• Subjects: Hypoxia-ischemia; Ischemic preconditioning; Mitochondrial K(ATP) channel; 마취과학
• ISSN: 2005-6419; 2005-7563

Background: A brief episode of cerebral ischemia confers transient ischemic tolerance to a subsequent ischemic challenge that is otherwise lethal to them. This study was purposed to evaluate the effect of mitochondrial adenosine triphosphate-sensitive potassium (K(ATP)) channel blocker on ischemic preconditioning in hypoxic-ischemic brain injury model of neonatal rat. Methods: Seven-day old Sprague-Dawley rat pups were used. The rats were divided into five groups; control group (n=91), pretreatment hypoxic preconditioning group (n=43), pretreatment ischemic preconditioning group (n=52), hypoxic preconditioning group (n=39), and ischemic preconditioning group (n=51). Rats in the pretreatment hypoxic preconditioning group and pretreatment ischemic preconditioning group were treated by an intraperitoneal injection with 5-hydroxydecanoate (60 mg/kg). Thirty minutes after injection, right common carotid artery was temporarily occluded for ten minutes in pretreatment ischemic preconditioning group. Rats in the pretreatment hypoxic preconditioning group and hypoxic preconditioning group underwent hypoxia (8% oxygen/92% nitrogen) for four hours. Twenty-four hours after the preconditioning, rats from all groups were exposed to right common carotid artery ligation followed by 2.5 hour hypoxia. On the 1st day after hypoxic-ischemic brain injury, terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling (TUNEL) reaction was evaluate as apoptotic markers and triphenyl tetrazolium chloride (TTC) was done to measure necrotic tissue. All rats were sacrificed 2 weeks after hypoxic-ischemia brain injury and the brains were examined for morphologic study. Results: There were no differenced in survival rate, infarct area, number of TUNEL positive cells and morphologic score either between hypoxic preconditioning group and pretreatment hypoxic preconditioning group or between ischemic preconditioning group and pretreatment ischemic preconditioning group. Conclusions: The results suggests that mitochondrial KATP channel blocker, 5-hydroxydecanoate, does not change hypoxic-ischemic preconditioning in the neonatal rat. (Korean J Anesthesiol 2009; 57: 729∼36)