Propofol의 청각뇌간반응과 뇌교 청신경핵 부위 절편에서의 시냅스후전류에 대한 효과 사이의 상관성 연구

• Published in: Korean journal of anesthesiology Vol. 56; no. 5; pp. 552 - 558
• Main Authors: 강봉진, Bong Jin Kang, 김석곤, Seok Kon Kim, 이관우, Gwan Woo Lee, 권민아, Min A Kwon, 송재격, Jae Gyok Song, 안승철, Seung Chul Ahn
• Format: Journal Article
• Language: Korean
• Published: 대한마취통증의학회 30.05.2009
• Subjects: Auditory brainstem response; Lateral superior olive; Nucleus of trapezoid body; Propofol; 마취과학
• ISSN: 2005-6419; 2005-7563

Background: Although there have been reports showing the changes of the auditory brainstem response (ABR) waves by propofol, no detailed studies have been done at the level of brainstem auditory circuit. So, we studied the effects of propofol on the postsynaptic currents of the medial nucleus of the trapezoid body (MNTB)-lateral superior olive (LSO) synapses by using the whole cell voltage clamp technique and we compared this data with that obtained by the ABR. Methods: 5 rats at postnatal (P) 15 days were used for the study of the ABR. After inducing deep anesthesia using xylazine 6 mg/kg and ketamine 25 mg/kg, the ABRs were recorded before and after intraperitoneal propofol injection (10 mg/kg) and the effects of propofol on the latencies of the I, III, and V waves and the I-III and III-V interwave intervals were evaluated. Rats that were aged under P11 were used in the voltage clamp experiments. After making brainstem slices, the postsynaptic currents (PSCs) elicited by MNTB stimulation were recorded at the LSO, and the changes of the PSCs by the bath application of propofol (100 μM) were monitored. Results: We found small, but statistically significant increases in the latencies of ABR waves III and V and the interwave intervals of I-III and III-V by propofol. However, no significant changes were observed in the glycinergic or glutamatergic PSCs of the MNTB-LSO synpases by the application of propofol (100 μM). Conclusions: Glycinergic or glutamatergic transmission of the MNTB-LSO synapses might not contribute to the propofol-induced changes of the ABR. (Korean J Anesthesiol 2009;56:552~8)