Hydrogen Peroxide로 유도된 산화성 간세포 손상에 대한 Propofol과 Etomidate의 효과

• Published in: Korean journal of anesthesiology Vol. 57; no. 3; pp. 331 - 336
• Main Authors: 이은호, Eun Ho Lee, 신진우, Jin Woo Shin, 윤선경, Sun Kyung Yoon, 손효정, Hyo Jung Son, 이지연, Ji Yeon Lee, 구승우, Seung Woo Ku, 김종욱, Joung Uk Kim, 이유미, Yu Mi Lee
• Format: Journal Article
• Language: Korean
• Published: 대한마취통증의학회(구 대한마취과학회) 30.09.2009; 대한마취통증의학회
• Subjects: Etomidate; Hydrogen peroxide; Lactate dehydrogenase; Oxidative stress; Propofol; 마취과학
• ISSN: 2005-6419; 2005-7563

Background: The present investigation was undertaken to evaluate the protective effect of propofol and etomidate against hydrogen peroxide (H2O2) induced oxidative damage in human hepatic SNU761 cells by measuring lactate dehydrogenase (LDH). Methods: The cell line of human hepatocellular carcinoma was grown for 24 hours in dissociated cell culture. They were divided into eight groups: negative control (NC) group with no drug administration, positive control (PC) group with H2O2 250 μM and other groups pretreated with propofol (P; 1, 10, 50 μM) or etomidate (ET; 1, 10, 50 μM) followed H2O2 administration. After 7 hours, cell death was assessed by morphology under the light microscope and quantified by measuring the LDH in the culture media. Results: In the light microscopic findings, the intact cells were increased in all three propofol groups compared to group PC. H2O2-induced LDH production was also significantly suppressed in all three propofol groups compared to group PC (P<0.001). There were no significant differences in the microscopic findings and LDH production between the etomidate groups and group PC. Conclusions: These results suggest that the propofol has protective effect on the hepatocyte against H2O2-induced oxidative stress. (Korean J Anesthesiol 2009;57:331∼6)